Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 813116..813636 Replicon chromosome
Accession NZ_CP059130
Organism Escherichia coli strain Ec55

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag H0X92_RS03850 Protein ID WP_001160964.1
Coordinates 813352..813636 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag H0X92_RS03845 Protein ID WP_000125566.1
Coordinates 813116..813349 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X92_RS03820 (808189) 808189..808476 + 288 WP_000203744.1 ferredoxin-like protein FixX -
H0X92_RS03825 (808535) 808535..809866 + 1332 WP_001183209.1 MFS transporter -
H0X92_RS03830 (809974) 809974..810504 + 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
H0X92_RS03835 (810497) 810497..812359 + 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
H0X92_RS03840 (812551) 812551..813030 + 480 WP_000624375.1 type 3 dihydrofolate reductase -
H0X92_RS03845 (813116) 813116..813349 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H0X92_RS03850 (813352) 813352..813636 + 285 WP_001160964.1 CcdB family protein Toxin
H0X92_RS03855 (813663) 813663..814511 - 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
H0X92_RS03860 (814518) 814518..814895 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
H0X92_RS03865 (814898) 814898..815719 - 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
H0X92_RS03870 (815716) 815716..816705 - 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
H0X92_RS03875 (816705) 816705..817991 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T168335 WP_001160964.1 NZ_CP059130:813352-813636 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T168335 NZ_CP075722:c4170429-4170256 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT168335 WP_000125566.1 NZ_CP059130:813116-813349 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT168335 NZ_CP075722:c4170753-4170457 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References