Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 123999..124642 Replicon plasmid pEc45_1
Accession NZ_CP059126
Organism Escherichia coli strain EC45

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag H0X93_RS24660 Protein ID WP_001034044.1
Coordinates 124226..124642 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag H0X93_RS24655 Protein ID WP_001261286.1
Coordinates 123999..124229 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X93_RS24635 (120630) 120630..121385 - 756 WP_000852146.1 replication initiation protein RepE -
H0X93_RS24640 (122107) 122107..122913 - 807 WP_000016970.1 site-specific integrase -
H0X93_RS24645 (122914) 122914..123219 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
H0X93_RS24650 (123221) 123221..123439 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
H0X93_RS24655 (123999) 123999..124229 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
H0X93_RS24660 (124226) 124226..124642 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
H0X93_RS24665 (124717) 124717..126282 + 1566 WP_001128474.1 AAA family ATPase -
H0X93_RS24670 (126267) 126267..127289 + 1023 WP_000361402.1 helicase UvrD -
H0X93_RS24675 (127543) 127543..128229 - 687 Protein_157 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / blaCTX-M-15 / aac(3)-IIa / tet(B) / catA1 / mph(A) / sitABCD iutA / iucD / iucC / iucB / iucA 1..146488 146488
- flank IS/Tn - - 127543..127890 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T168328 WP_001034044.1 NZ_CP059126:124226-124642 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T168328 NZ_CP075722:c2382721-2382545 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT168328 WP_001261286.1 NZ_CP059126:123999-124229 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT168328 NZ_CP075722:c2382499-2382083 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References