Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 4374719..4375305 Replicon chromosome
Accession NZ_CP059125
Organism Escherichia coli strain EC45

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag H0X93_RS21205 Protein ID WP_042014162.1
Coordinates 4375075..4375305 (-) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag H0X93_RS21200 Protein ID WP_000593555.1
Coordinates 4374719..4375078 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X93_RS21175 (4370851) 4370851..4371795 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
H0X93_RS21180 (4371792) 4371792..4372625 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
H0X93_RS21185 (4372625) 4372625..4373389 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
H0X93_RS21190 (4373386) 4373386..4374192 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
H0X93_RS21195 (4374198) 4374198..4374599 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
H0X93_RS21200 (4374719) 4374719..4375078 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
H0X93_RS21205 (4375075) 4375075..4375305 - 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
H0X93_RS21210 (4375409) 4375409..4376533 - 1125 WP_001314210.1 ABC transporter permease -
H0X93_RS21215 (4376533) 4376533..4379268 - 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T168318 WP_042014162.1 NZ_CP059125:c4375305-4375075 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T168318 NZ_CP075722:650382-650696 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT168318 WP_000593555.1 NZ_CP059125:c4375078-4374719 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT168318 NZ_CP075722:650693-651109 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References