Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3790053..3790707 Replicon chromosome
Accession NZ_CP059125
Organism Escherichia coli strain EC45

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag H0X93_RS18245 Protein ID WP_000244777.1
Coordinates 3790053..3790460 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H0X93_RS18250 Protein ID WP_000354046.1
Coordinates 3790441..3790707 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X93_RS18225 (3786010) 3786010..3787743 - 1734 WP_000813212.1 single-stranded-DNA-specific exonuclease RecJ -
H0X93_RS18230 (3787749) 3787749..3788459 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H0X93_RS18235 (3788484) 3788484..3789380 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H0X93_RS18240 (3789492) 3789492..3790013 + 522 WP_001055874.1 flavodoxin FldB -
H0X93_RS18245 (3790053) 3790053..3790460 - 408 WP_000244777.1 protein YgfX Toxin
H0X93_RS18250 (3790441) 3790441..3790707 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H0X93_RS18255 (3790950) 3790950..3791930 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H0X93_RS18260 (3792007) 3792007..3792666 - 660 WP_000250274.1 hemolysin III family protein -
H0X93_RS18265 (3792830) 3792830..3793141 - 312 WP_001679467.1 N(4)-acetylcytidine aminohydrolase -
H0X93_RS18270 (3793186) 3793186..3794619 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
H0X93_RS18275 (3794676) 3794676..3795419 - 744 Protein_3580 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T168314 WP_000244777.1 NZ_CP059125:c3790460-3790053 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T168314 NZ_CP075719:7032-7292 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT168314 WP_000354046.1 NZ_CP059125:c3790707-3790441 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT168314 NZ_CP075719:7282-7533 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References