Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1108210..1108828 Replicon chromosome
Accession NZ_CP059125
Organism Escherichia coli strain EC45

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H0X93_RS05270 Protein ID WP_001291435.1
Coordinates 1108210..1108428 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H0X93_RS05275 Protein ID WP_000344800.1
Coordinates 1108454..1108828 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X93_RS05235 (1103499) 1103499..1104071 + 573 WP_000779831.1 YbaY family lipoprotein -
H0X93_RS05240 (1104102) 1104102..1104413 - 312 WP_000409911.1 MGMT family protein -
H0X93_RS05250 (1104792) 1104792..1105145 + 354 WP_000878140.1 DUF1428 domain-containing protein -
H0X93_RS05255 (1105187) 1105187..1106737 - 1551 WP_001372022.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H0X93_RS05260 (1106901) 1106901..1107371 - 471 WP_000136192.1 YlaC family protein -
H0X93_RS05265 (1107487) 1107487..1108038 - 552 WP_000102568.1 maltose O-acetyltransferase -
H0X93_RS05270 (1108210) 1108210..1108428 - 219 WP_001291435.1 HHA domain-containing protein Toxin
H0X93_RS05275 (1108454) 1108454..1108828 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H0X93_RS05280 (1109374) 1109374..1112523 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
H0X93_RS05285 (1112546) 1112546..1113739 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T168300 WP_001291435.1 NZ_CP059125:c1108428-1108210 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T168300 NZ_CP075716:2032411-2032689 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT168300 WP_000344800.1 NZ_CP059125:c1108828-1108454 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT168300 NZ_CP075716:2032148-2032408 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References