Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 148195..148720 Replicon plasmid pEc42_1
Accession NZ_CP059120
Organism Escherichia coli strain Ec42

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H0X94_RS24665 Protein ID WP_001159871.1
Coordinates 148195..148500 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag H0X94_RS24670 Protein ID WP_000813630.1
Coordinates 148502..148720 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X94_RS24650 (144157) 144157..145323 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
H0X94_RS24655 (145911) 145911..146666 - 756 WP_000852146.1 replication initiation protein RepE -
H0X94_RS24660 (147388) 147388..148194 - 807 WP_000016970.1 site-specific integrase -
H0X94_RS24665 (148195) 148195..148500 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H0X94_RS24670 (148502) 148502..148720 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H0X94_RS24675 (149280) 149280..149510 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
H0X94_RS24680 (149507) 149507..149923 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
H0X94_RS24685 (149998) 149998..151563 + 1566 WP_001128474.1 AAA family ATPase -
H0X94_RS24690 (151548) 151548..152570 + 1023 WP_000361402.1 helicase UvrD -
H0X94_RS24695 (152824) 152824..153510 - 687 Protein_195 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / tet(B) / qacE / aadA5 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / sitABCD iutA / iucD / iucC / iucB / iucA 1..171769 171769
- flank IS/Tn - - 152824..153171 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T168291 WP_001159871.1 NZ_CP059120:c148500-148195 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T168291 NZ_CP075703:c3066-2716 [Escherichia coli]
ATGTGGACTGTTTATTTTGGCAGATTATTTGATGAATGGTTTGAAGAGCAGGAACTAGCCCTAAAACGAAAAGTGCTGGC
TGAACTTAAACATCTTGAAGAGTTTGGCCCCTCTCTTTCACGGCCACATGCAGATACAGTGAAAGGGTCACGTCATAAGA
ATATGAAGGAACTACGTATACAATATGAGGGACACCCAATCAGGGCATTCTTTGCATTTGATCCAATTAGACAAGCAATC
ATTCTTTGCGCTGGCGACAAAAGTAATGATAAGAAATTTTACGACAGAATGATTCGTATTGCTGACGAAGAATTTTCCAC
TCACTTAGCCGAACTGGAGGGCAAAAAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT168291 WP_000813630.1 NZ_CP059120:c148720-148502 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT168291 NZ_CP075703:c2719-2420 [Escherichia coli]
ATGAGAACATTAGAAGAAGTAATTGCGGCTCAATCACCTGAAAGTCAGGCGATCATCAAAGCTATGGCTGATGAGATGAT
TCTGGAAACAGGGCTGCAAATGATGCGTGAAGAATTGCAGCTTTCACAAAAACAGGTTGCTGATGCAATGGGAATCAGTC
AGCCCGCTGTAACTCAACTGGAACAGCGCGGAAATGATTTGAAGCTGGCAACGTTAAAGCGTTACGTTGAAGCTATGGGA
GGAAAGTTGAGCCTAGATGTTGAACTCCCTACCGGAAAGCGAGTGGCCTTTAACATTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References