Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4471521..4472018 Replicon chromosome
Accession NZ_CP059119
Organism Escherichia coli strain Ec42

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag H0X94_RS21585 Protein ID WP_001173343.1
Coordinates 4471521..4471694 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag H0X94_RS21590 Protein ID WP_000398619.1
Coordinates 4471722..4472018 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X94_RS21575 (4468191) 4468191..4469648 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
H0X94_RS21580 (4469885) 4469885..4471402 + 1518 WP_001295074.1 lysine--tRNA ligase -
H0X94_RS21585 (4471521) 4471521..4471694 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
H0X94_RS21590 (4471722) 4471722..4472018 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
H0X94_RS21595 (4472245) 4472245..4472517 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
H0X94_RS21600 (4472529) 4472529..4472759 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
H0X94_RS21605 (4472940) 4472940..4474571 + 1632 WP_001216477.1 sensor histidine kinase -
H0X94_RS21610 (4474568) 4474568..4475287 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T168283 WP_001173343.1 NZ_CP059119:c4471694-4471521 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T168283 NZ_CP075697:c4745293-4745186 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTAGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT168283 WP_000398619.1 NZ_CP059119:c4472018-4471722 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT168283 NZ_CP075697:4745340-4745406 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References