Detailed information of TA system TA168277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2528788..2529426
• Replicon: chromosome
• Accession: NZ_CP059119
• Organism: Escherichia coli strain Ec42

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: H0X94_RS12185
• Protein ID: WP_001447010.1
• Coordinates: 2529250..2529426 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H0X94_RS12180
• Protein ID: WP_001270286.1
• Coordinates: 2528788..2529204 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0X94_RS12160 (2523940)	2523940..2524881	-	942	WP_001251304.1	ABC transporter permease	-
H0X94_RS12165 (2524882)	2524882..2525895	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
H0X94_RS12170 (2525913)	2525913..2527058	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
H0X94_RS12175 (2527303)	2527303..2528709	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
H0X94_RS12180 (2528788)	2528788..2529204	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H0X94_RS12185 (2529250)	2529250..2529426	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H0X94_RS12190 (2529648)	2529648..2529878	+	231	WP_000494244.1	YncJ family protein	-
H0X94_RS12195 (2529970)	2529970..2531931	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H0X94_RS12200 (2532004)	2532004..2532540	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
H0X94_RS12205 (2532632)	2532632..2533807	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T168277 WP_001447010.1 NZ_CP059119:c2529426-2529250 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T168277 NZ_CP075697:c3366774-3366310 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT168277 WP_001270286.1 NZ_CP059119:c2529204-2528788 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT168277 NZ_CP075697:c3367109-3366774 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8