Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1044756..1045410 Replicon chromosome
Accession NZ_CP059119
Organism Escherichia coli strain Ec42

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag H0X94_RS05025 Protein ID WP_000244781.1
Coordinates 1044756..1045163 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H0X94_RS05030 Protein ID WP_000354046.1
Coordinates 1045144..1045410 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0X94_RS05005 (1040713) 1040713..1042446 - 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -
H0X94_RS05010 (1042452) 1042452..1043162 - 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H0X94_RS05015 (1043187) 1043187..1044083 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H0X94_RS05020 (1044195) 1044195..1044716 + 522 WP_001521233.1 flavodoxin FldB -
H0X94_RS05025 (1044756) 1044756..1045163 - 408 WP_000244781.1 protein YgfX Toxin
H0X94_RS05030 (1045144) 1045144..1045410 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H0X94_RS05035 (1045653) 1045653..1046633 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
H0X94_RS05040 (1046710) 1046710..1047369 - 660 WP_042345878.1 hemolysin III family protein -
H0X94_RS05045 (1047533) 1047533..1047844 - 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
H0X94_RS05050 (1047889) 1047889..1049322 + 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T168265 WP_000244781.1 NZ_CP059119:c1045163-1044756 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T168265 NZ_CP075697:c227465-227289 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT168265 WP_000354046.1 NZ_CP059119:c1045410-1045144 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT168265 NZ_CP075697:c227243-226827 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGAAATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References