Detailed information of TA system TA168245

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 22299..22950
• Replicon: plasmid pAR1aB
• Accession: NZ_CP059088
• Organism: Pantoea agglomerans strain AR1a

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: H0Z11_RS00545
• Protein ID: WP_137229084.1
• Coordinates: 22777..22950 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H0Z11_RS00540
• Protein ID: WP_137229086.1
• Coordinates: 22299..22706 (-)
• Length: 136 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0Z11_RS00530 (H0Z11_00530)	18050..19165	-	1116	WP_207874171.1	metallophosphoesterase	-
H0Z11_RS00535 (H0Z11_00535)	19403..21730	-	2328	WP_207874173.1	FdhF/YdeP family oxidoreductase	-
H0Z11_RS00540 (H0Z11_00540)	22299..22706	-	408	WP_137229086.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H0Z11_RS00545 (H0Z11_00545)	22777..22950	-	174	WP_137229084.1	type II toxin-antitoxin system HicA family toxin	Toxin
H0Z11_RS00550 (H0Z11_00550)	23580..23927	-	348	WP_003850685.1	HNH nuclease YajD	-
H0Z11_RS00555 (H0Z11_00555)	24048..24329	-	282	WP_205317936.1	helix-turn-helix transcriptional regulator	-
H0Z11_RS00560 (H0Z11_00560)	24319..24639	-	321	WP_207874175.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H0Z11_RS00565 (H0Z11_00565)	24963..25130	-	168	WP_009093121.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..160336	160336

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-55)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6538.66 Da
• Isoelectric Point: 10.7838

>T168245 WP_137229084.1 NZ_CP059088:c22950-22777 [Pantoea agglomerans]
MNSAQLMKLLKRNGWVLDRVNGSHHIFVKEGVDHHITVPHPRKDLAKGTLQDILKNM

Nucleotide

• Length: 174 bp

>T168245 NZ_CP075666:2080-2367 [Escherichia coli]
ATGACTTATACAGTAAAATTCAGGGATGATGCGCTCAAAGAATGGCTGAAACTGGATAAATCCATTCAACAGCAGTTTGC
GAAAAAACTAAAGAAATGCAGCGAAAATCCTCATATTCCTTCCGCAAAACTGCGTGGATTAAAAGACTGCTACAAAATCA
AACTTCGCGCATCGGGGTTTCGTCTGGTCTATCAGGTTATTGATGACATGCTAATTATTGCGGTTGTCGCGGTAGGTAAA
CGTGAACGTAGTAACGTTTATAATCTCGCCAGTGAGAGAATGAGATAG

Antitoxin

Protein

• Length: 136 a.a.
• Molecular weight: 14965.92 Da
• Isoelectric Point: 4.9464

>AT168245 WP_137229086.1 NZ_CP059088:c22706-22299 [Pantoea agglomerans]
MKFPIYLHKAEGGSFSGFVPDIKGCYFAGDNIDDTIADAFSAIDAHLEFQAEKGKALPTAMPVEAHFDDENCQGGYWAFV
DIDLSKYDGKAVKLNITLPQNLLKKIDSYVELHKDFGSRSGFIAEIARRELQKTA

Nucleotide

• Length: 408 bp

>AT168245 NZ_CP075666:1832-2083 [Escherichia coli]
ATGCCTAACATCATTCTGTCTGACACCAGTGCAAGTGTCAGTGAACTGAAAAAAAATCCTATGGCTACAGTCAGTGCCGG
AGATGGTTATCCTGTCGCCATTCTTAACCGGAATCAGCCGGCATTCTACTGTGTGCCTGCGGAACTCTATGAAAGGATGC
TTGATGCGCTGGATGACCAGGAACTGGTGAAACTGGTTACTGAACGCAGCAATCAGCCACTGCATGATGTGGACCTGGAC
AGTTATTTATGA