Detailed information of TA system TA168244

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 79009..79562
• Replicon: plasmid pAR1aD
• Accession: NZ_CP059087
• Organism: Pantoea agglomerans strain AR1a

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H0Z11_RS00400
• Protein ID: WP_031594618.1
• Coordinates: 79248..79562 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A7X2STP4
• Locus tag: H0Z11_RS00395
• Protein ID: WP_033781302.1
• Coordinates: 79009..79245 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0Z11_RS00365 (H0Z11_00365)	74482..74856	+	375	WP_010260804.1	fluoride efflux transporter CrcB	-
H0Z11_RS00370 (H0Z11_00370)	74975..75202	-	228	Protein_74	DNA polymerase V subunit UmuC	-
H0Z11_RS00375 (H0Z11_00375)	75202..75582	-	381	WP_187495410.1	peptidase	-
H0Z11_RS00380 (H0Z11_00380)	75720..76400	+	681	Protein_76	SOS response-associated peptidase family protein	-
H0Z11_RS00385 (H0Z11_00385)	76857..77306	+	450	WP_072124008.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
H0Z11_RS00390 (H0Z11_00390)	77315..78589	+	1275	WP_134162036.1	translesion error-prone DNA polymerase V subunit UmuC	-
H0Z11_RS00395 (H0Z11_00395)	79009..79245	+	237	WP_033781302.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H0Z11_RS00400 (H0Z11_00400)	79248..79562	+	315	WP_031594618.1	CcdB family protein	Toxin
H0Z11_RS00405 (H0Z11_00405)	79705..79953	-	249	WP_227024379.1	hypothetical protein	-
H0Z11_RS00410 (H0Z11_00410)	80031..80234	-	204	WP_115763907.1	plasmid partition protein ParG	-
H0Z11_RS00415 (H0Z11_00415)	80231..80854	-	624	WP_031594621.1	ParA family partition ATPase	-
H0Z11_RS00420 (H0Z11_00420)	81117..81911	-	795	WP_033781298.1	site-specific integrase	-
H0Z11_RS00425 (H0Z11_00425)	81904..82452	-	549	WP_134162031.1	hypothetical protein	-
H0Z11_RS00430 (H0Z11_00430)	82550..82999	-	450	WP_033781295.1	hypothetical protein	-
H0Z11_RS00435 (H0Z11_00435)	83083..84018	-	936	WP_109653962.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90466	90466

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (9-78)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11658.60 Da
• Isoelectric Point: 5.8036

>T168244 WP_031594618.1 NZ_CP059087:79248-79562 [Pantoea agglomerans]
MQFTVYSNTGKSAVYPLLLDVTSDIIGQLNRRIVIPLLPLEKYPSSTRPERLVPLIRLTDDKEYAVMTHEMASIPTRALG
AVFCDSSLYRTEIKAAIDFLLDGI

Nucleotide

• Length: 315 bp

>T168244 NZ_CP075665:5293568-5293966 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8966.90 Da
• Isoelectric Point: 4.6234

>AT168244 WP_033781302.1 NZ_CP059087:79009-79245 [Pantoea agglomerans]
MAITQRATQSVTMTVERDLLSRAREAGINLSATLTTALDAELRQYEAKKWQEENSEALEALNRFHDEHGCFSDEYRTF

Nucleotide

• Length: 237 bp

>AT168244 NZ_CP075665:5293341-5293568 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7X2STP4