Detailed information of TA system TA168243

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3387274..3387891
• Replicon: chromosome
• Accession: NZ_CP059084
• Organism: Pantoea ananatis strain OC5a

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: D4GLR0
• Locus tag: H0Z12_RS17105
• Protein ID: WP_013024903.1
• Coordinates: 3387676..3387891 (+)
• Length: 72 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A8A4KA49
• Locus tag: H0Z12_RS17100
• Protein ID: WP_015700827.1
• Coordinates: 3387274..3387651 (+)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0Z12_RS17070 (H0Z12_17075)	3383560..3383814	+	255	WP_014606427.1	type B 50S ribosomal protein L31	-
H0Z12_RS17075 (H0Z12_17080)	3383826..3383966	+	141	WP_014606428.1	type B 50S ribosomal protein L36	-
H0Z12_RS17080 (H0Z12_17085)	3384026..3384904	-	879	WP_013024908.1	metal ABC transporter substrate-binding protein	-
H0Z12_RS17085 (H0Z12_17090)	3384917..3385756	-	840	WP_013024907.1	metal ABC transporter permease	-
H0Z12_RS17090 (H0Z12_17095)	3385753..3386421	-	669	WP_014606429.1	ABC transporter ATP-binding protein	-
H0Z12_RS17095 (H0Z12_17100)	3386774..3387127	+	354	WP_013024905.1	hypothetical protein	-
H0Z12_RS17100 (H0Z12_17105)	3387274..3387651	+	378	WP_015700827.1	Hha toxicity modulator TomB	Antitoxin
H0Z12_RS17105 (H0Z12_17110)	3387676..3387891	+	216	WP_013024903.1	HHA domain-containing protein	Toxin
H0Z12_RS17115 (H0Z12_17120)	3388289..3388600	+	312	WP_015700828.1	MGMT family protein	-
H0Z12_RS17120 (H0Z12_17125)	3388649..3389206	-	558	WP_013024901.1	YbaY family lipoprotein	-
H0Z12_RS17125 (H0Z12_17130)	3389399..3390262	+	864	WP_013024900.1	acyl-CoA thioesterase II	-
H0Z12_RS17130 (H0Z12_17135)	3390309..3391598	-	1290	WP_072137846.1	ammonium transporter AmtB	-
H0Z12_RS17135 (H0Z12_17140)	3391633..3391971	-	339	WP_013024898.1	P-II family nitrogen regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	acrA / acrB / gmhA/lpcA	3341678..3588439	246761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (12-68)

Antitoxin

• Biofilm formation regulator YbaJ (1-114)

Sequences

Toxin

Protein

• Length: 72 a.a.
• Molecular weight: 8552.98 Da
• Isoelectric Point: 9.4825

>T168243 WP_013024903.1 NZ_CP059084:3387676-3387891 [Pantoea ananatis]
MNKTLTKTDYLMRLRRCRSIDTLERVIEKNKYELPDEELAVFYSAADHRLAELTMNKLYDKVPVSVWKFVR

Nucleotide

• Length: 216 bp

>T168243 NZ_CP075665:c4950385-4950107 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 14722.35 Da
• Isoelectric Point: 4.3976

>AT168243 WP_015700827.1 NZ_CP059084:3387274-3387651 [Pantoea ananatis]
MDEYSPKRHDIAQLKYLCENLFDDSMATLTDSHHGWVNDPTAESNLQLNDLIEHIASFTMNYKIKHVEDEALITQIDEYL
DDTFMLFSNYSVNTQDLQRWQRSAKRLFNVFTEECAFLQQPSHSL

Nucleotide

• Length: 378 bp

>AT168243 NZ_CP075665:c4950648-4950388 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA