Detailed information of TA system TA168229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 23042..23949
• Replicon: plasmid pOC5aA
• Accession: NZ_CP059083
• Organism: Pantoea ananatis strain OC5a

Toxin (Protein)

• Gene name: -
• Uniprot ID: D4GET9
• Locus tag: H0Z12_RS00070
• Protein ID: WP_013027861.1
• Coordinates: 23042..23509 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A8A4JZ23
• Locus tag: H0Z12_RS00075
• Protein ID: WP_014598335.1
• Coordinates: 23506..23949 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0Z12_RS00050 (H0Z12_00050)	18428..18661	-	234	WP_013027857.1	DUF333 domain-containing protein	-
H0Z12_RS00055 (H0Z12_00055)	18749..19321	+	573	WP_014598338.1	NADPH-dependent FMN reductase	-
H0Z12_RS00060 (H0Z12_00060)	19383..20318	-	936	WP_013027859.1	flagellin	-
H0Z12_RS00065 (H0Z12_00065)	21003..22397	-	1395	WP_014333136.1	flagellar filament capping protein FliD	-
H0Z12_RS00070 (H0Z12_00070)	23042..23509	-	468	WP_013027861.1	RES family NAD+ phosphorylase	Toxin
H0Z12_RS00075 (H0Z12_00075)	23506..23949	-	444	WP_014598335.1	DUF2384 domain-containing protein	Antitoxin
H0Z12_RS00080 (H0Z12_00080)	24335..24559	+	225	WP_013027863.1	KTSC domain-containing protein	-
H0Z12_RS00085 (H0Z12_00085)	24713..25408	+	696	WP_019106864.1	hypothetical protein	-
H0Z12_RS00090 (H0Z12_00090)	25483..26172	-	690	WP_014598333.1	HTH-type transcriptional regulator RutR	-
H0Z12_RS00095 (H0Z12_00095)	26571..27662	+	1092	WP_013027866.1	pyrimidine utilization protein A	-
H0Z12_RS00100 (H0Z12_00100)	27659..28411	+	753	WP_019105823.1	pyrimidine utilization protein B	-
H0Z12_RS00105 (H0Z12_00105)	28437..28823	+	387	WP_013027868.1	pyrimidine utilization protein C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fliC / fliC	1..305982	305982

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (2-145)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-89)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17207.70 Da
• Isoelectric Point: 5.1570

>T168229 WP_013027861.1 NZ_CP059083:c23509-23042 [Pantoea ananatis]
MKLYRMTKTRYLGSAWSGFGAREGGGRWNSVGVSMVYASETASLTMLETLIHLQSASVLDFFTLMSIDVPDRLIEWIDIK
QLPDDWAAPEAPAALRLFGDAWIQSGGSVALRVPSALSPVESNYLLNPEHPEFSAIVRQAVNIPFLFDARFSRQV

Nucleotide

• Length: 468 bp

>T168229 NZ_CP075665:1407171-1407278 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16375.69 Da
• Isoelectric Point: 7.0208

>AT168229 WP_014598335.1 NZ_CP059083:c23949-23506 [Pantoea ananatis]
MKQFTLPVSAAEPDRLWQFAGLSSADGVMLMDQIDDGLDGTVASRIVTWAQISQAELRRMTGIAGSTFTRGVKSRFTAEQ
SERLVRFIRVMDRAVELFEGDKEKARQWLNEPARALGNKKPADLVASETGAYEVLKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT168229 NZ_CP075665:c1407124-1407058 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT