Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 23042..23949 Replicon plasmid pOC5aA
Accession NZ_CP059083
Organism Pantoea ananatis strain OC5a

Toxin (Protein)


Gene name - Uniprot ID D4GET9
Locus tag H0Z12_RS00070 Protein ID WP_013027861.1
Coordinates 23042..23509 (-) Length 156 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A8A4JZ23
Locus tag H0Z12_RS00075 Protein ID WP_014598335.1
Coordinates 23506..23949 (-) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0Z12_RS00050 (H0Z12_00050) 18428..18661 - 234 WP_013027857.1 DUF333 domain-containing protein -
H0Z12_RS00055 (H0Z12_00055) 18749..19321 + 573 WP_014598338.1 NADPH-dependent FMN reductase -
H0Z12_RS00060 (H0Z12_00060) 19383..20318 - 936 WP_013027859.1 flagellin -
H0Z12_RS00065 (H0Z12_00065) 21003..22397 - 1395 WP_014333136.1 flagellar filament capping protein FliD -
H0Z12_RS00070 (H0Z12_00070) 23042..23509 - 468 WP_013027861.1 RES family NAD+ phosphorylase Toxin
H0Z12_RS00075 (H0Z12_00075) 23506..23949 - 444 WP_014598335.1 DUF2384 domain-containing protein Antitoxin
H0Z12_RS00080 (H0Z12_00080) 24335..24559 + 225 WP_013027863.1 KTSC domain-containing protein -
H0Z12_RS00085 (H0Z12_00085) 24713..25408 + 696 WP_019106864.1 hypothetical protein -
H0Z12_RS00090 (H0Z12_00090) 25483..26172 - 690 WP_014598333.1 HTH-type transcriptional regulator RutR -
H0Z12_RS00095 (H0Z12_00095) 26571..27662 + 1092 WP_013027866.1 pyrimidine utilization protein A -
H0Z12_RS00100 (H0Z12_00100) 27659..28411 + 753 WP_019105823.1 pyrimidine utilization protein B -
H0Z12_RS00105 (H0Z12_00105) 28437..28823 + 387 WP_013027868.1 pyrimidine utilization protein C -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - fliC / fliC 1..305982 305982


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(2-145)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(31-89)


Sequences

Toxin        


Download         Length: 156 a.a.        Molecular weight: 17207.70 Da        Isoelectric Point: 5.1570

>T168229 WP_013027861.1 NZ_CP059083:c23509-23042 [Pantoea ananatis]
MKLYRMTKTRYLGSAWSGFGAREGGGRWNSVGVSMVYASETASLTMLETLIHLQSASVLDFFTLMSIDVPDRLIEWIDIK
QLPDDWAAPEAPAALRLFGDAWIQSGGSVALRVPSALSPVESNYLLNPEHPEFSAIVRQAVNIPFLFDARFSRQV

Download         Length: 468 bp

>T168229 NZ_CP075665:1407171-1407278 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16375.69 Da        Isoelectric Point: 7.0208

>AT168229 WP_014598335.1 NZ_CP059083:c23949-23506 [Pantoea ananatis]
MKQFTLPVSAAEPDRLWQFAGLSSADGVMLMDQIDDGLDGTVASRIVTWAQISQAELRRMTGIAGSTFTRGVKSRFTAEQ
SERLVRFIRVMDRAVELFEGDKEKARQWLNEPARALGNKKPADLVASETGAYEVLKLITRLEHGVYS

Download         Length: 444 bp

>AT168229 NZ_CP075665:c1407124-1407058 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References