Detailed information of TA system TA168227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 5150606..5151723
• Replicon: chromosome
• Accession: NZ_CP059082
• Organism: Halomonas titanicae strain SOB56

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HZS52_RS23675
• Protein ID: WP_190373858.1
• Coordinates: 5150606..5151355 (-)
• Length: 250 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7Z0LWE6
• Locus tag: HZS52_RS23680
• Protein ID: WP_022519610.1
• Coordinates: 5151352..5151723 (-)
• Length: 124 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS52_RS23650	5146425..5146769	+	345	WP_138799703.1	DUF952 domain-containing protein	-
HZS52_RS23655	5147110..5148696	+	1587	WP_190373857.1	flavin monoamine oxidase family protein	-
HZS52_RS23660	5148710..5149111	+	402	WP_138799702.1	cytochrome c	-
HZS52_RS23665	5149170..5149664	+	495	WP_022519607.1	RidA family protein	-
HZS52_RS23670	5149764..5150528	-	765	WP_138799701.1	prolyl oligopeptidase family serine peptidase	-
HZS52_RS23675	5150606..5151355	-	750	WP_190373858.1	RES family NAD+ phosphorylase	Toxin
HZS52_RS23680	5151352..5151723	-	372	WP_022519610.1	DUF2384 domain-containing protein	Antitoxin
HZS52_RS23685	5151951..5152727	+	777	WP_027957215.1	aspartate/glutamate racemase family protein	-
HZS52_RS23690	5152744..5153175	-	432	WP_022519612.1	transcriptional regulator	-
HZS52_RS23695	5153268..5154995	-	1728	WP_190373859.1	mechanosensitive ion channel	-
HZS52_RS23700	5155185..5155718	-	534	WP_190373860.1	GDYXXLXY domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (62-206)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (9-68)

Sequences

Toxin

Protein

• Length: 250 a.a.
• Molecular weight: 27804.57 Da
• Isoelectric Point: 5.8853

>T168227 WP_190373858.1 NZ_CP059082:c5151355-5150606 [Halomonas titanicae]
VSVWEACGGARNIKPISGTLYRLVESQEQVATLSYVDTLEEQEMLEALLETAKPPYPEQERDLHYLLKTPFRYPPLKWGS
RFGRVFEPSIFYGGKGIATTLAESAYYRLVFWYSMEGDPVTASLRSEHTLFSVDYAAKRGVRLYTGFFVDHLAVLKHPVD
YAPCQQLGSAMRGAGVEAFEYASARDIQGGACVGLFTPDALAQHEPADMRPWLCVTSAAQVSFKRKDASGVYTYHLDDFL
HQGVLPFPA

Nucleotide

• Length: 750 bp

>T168227 NZ_CP075665:1045391-1046713 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CAGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTAACGTTATTACCCGAAAA
TGAAACCATAACGCGCCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGGCTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTTAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAATTCCAGGTCTTCCAGTGGTTGATTGGCGCAACGGACGGTCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTTTGGCGACAGCAAAGGTGCTGAGATTCCCGGAAGTGCAGATGCATGAAATCCTAAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCATGGACGGTTAAGCCGAGAATACGGTAGTAAGTGA

Antitoxin

Protein

• Length: 124 a.a.
• Molecular weight: 13447.81 Da
• Isoelectric Point: 11.0983

>AT168227 WP_022519610.1 NZ_CP059082:c5151723-5151352 [Halomonas titanicae]
MPALTQTKPTPEMVLAKAVLRAAEQLGLSQSDLAEVLGVHRTTITQLKKSLKLDPNSKQGELALLLIRLARALFALTGGD
QTWIQHFMRTSNRVTGGIPAEQVTRLEGLFAVLRFVDGMRGKV

Nucleotide

• Length: 372 bp

>AT168227 NZ_CP075665:1045125-1045391 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGCCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATTGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACACTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A7Z0LWE6