Detailed information of TA system TA168225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-couple_hipB
• Location: 4627735..4628387
• Replicon: chromosome
• Accession: NZ_CP059082
• Organism: Halomonas titanicae strain SOB56

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7H1VA24
• Locus tag: HZS52_RS21245
• Protein ID: WP_027959641.1
• Coordinates: 4628136..4628387 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HZS52_RS21240
• Protein ID: WP_022520397.1
• Coordinates: 4627735..4628097 (-)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS52_RS21215	4624221..4625120	+	900	WP_190373612.1	LysR family transcriptional regulator	-
HZS52_RS21220	4625198..4625362	+	165	WP_155830865.1	hypothetical protein	-
HZS52_RS21225	4625524..4626180	-	657	WP_027959639.1	hypothetical protein	-
HZS52_RS21230	4626850..4627221	+	372	WP_190373613.1	DUF1232 domain-containing protein	-
HZS52_RS21235	4627401..4627547	+	147	WP_190374955.1	helix-turn-helix domain-containing protein	-
HZS52_RS21240	4627735..4628097	-	363	WP_022520397.1	helix-turn-helix transcriptional regulator	Antitoxin
HZS52_RS21245	4628136..4628387	-	252	WP_027959641.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZS52_RS21250	4629604..4629963	+	360	WP_190373614.1	hypothetical protein	-
HZS52_RS21255	4630005..4630763	-	759	WP_190374956.1	DNA adenine methylase	-
HZS52_RS21260	4630898..4631941	-	1044	WP_190373615.1	phage portal protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4576767..4672659	95892

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-78)

Antitoxin

• Cro/C1-type helix-turn-helix domain (65-119)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9572.12 Da
• Isoelectric Point: 10.7166

>T168225 WP_027959641.1 NZ_CP059082:c4628387-4628136 [Halomonas titanicae]
MKTITYRKSALKVLQRMPRPESKRIQGKIKAYAEDPESQRNNVKALKGSDYIRLRVGDWRVIIDERGDVLDIINIGARGA
IYQ

Nucleotide

• Length: 252 bp

>T168225 NZ_CP075665:485962-486216 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 12848.52 Da
• Isoelectric Point: 4.3074

>AT168225 WP_022520397.1 NZ_CP059082:c4628097-4627735 [Halomonas titanicae]
MSAPQIIQTPTGERLVVLPEQEYHALLARLEDQDDIDAANAVVARGEESFPAAVVDALLDGVAPVKVYREYRGLRAGELA
EKAGISQGYLSEIESGKKSGSLSVLTRIAAALDVELRDIT

Nucleotide

• Length: 363 bp

>AT168225 NZ_CP075665:485714-485965 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7H1VA24