Detailed information of TA system TA168224

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 2797487..2798102
• Replicon: chromosome
• Accession: NZ_CP059082
• Organism: Halomonas titanicae strain SOB56

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A1G8NTB1
• Locus tag: HZS52_RS12890
• Protein ID: WP_022522690.1
• Coordinates: 2797806..2798102 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HZS52_RS12885
• Protein ID: WP_022522689.1
• Coordinates: 2797487..2797801 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS52_RS12870	2792643..2796269	-	3627	WP_190372918.1	exodeoxyribonuclease V subunit gamma	-
HZS52_RS12875	2796446..2796877	+	432	WP_022522688.1	DUF2384 domain-containing protein	-
HZS52_RS12880	2796874..2797392	+	519	WP_009287632.1	RES domain-containing protein	-
HZS52_RS12885	2797487..2797801	-	315	WP_022522689.1	putative addiction module antidote protein	Antitoxin
HZS52_RS12890	2797806..2798102	-	297	WP_022522690.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HZS52_RS12895	2798275..2799957	+	1683	WP_009287633.1	dihydroxy-acid dehydratase	-
HZS52_RS12900	2800070..2801263	+	1194	WP_190372919.1	chromate efflux transporter	-
HZS52_RS12905	2801390..2801662	-	273	WP_008958942.1	XRE family transcriptional regulator	-
HZS52_RS12910	2801882..2802397	+	516	WP_022522693.1	5-(carboxyamino)imidazole ribonucleotide mutase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2690300..2820333	130033

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-96)

Antitoxin

• Uncharacterized protein HI1420 (8-94)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11045.95 Da
• Isoelectric Point: 10.2250

>T168224 WP_022522690.1 NZ_CP059082:c2798102-2797806 [Halomonas titanicae]
MIELIKTDVFDRWLRELRDIRARAKVEARIRRLSLGNPGDVKPVGKGVSELRIPYGPGYRVYYITKGPVIVVLLCGGDKA
TQSHDIELAKAIAAQWKE

Nucleotide

• Length: 297 bp

>T168224 NZ_CP075665:485714-485965 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11074.48 Da
• Isoelectric Point: 4.4217

>AT168224 WP_022522689.1 NZ_CP059082:c2797801-2797487 [Halomonas titanicae]
MTVPNVTFSHYDTADYLQTEEDIAAYLDAVMEENDPALLAAALGDIARARNMSQLAKDVGMSREGLYKALSGEGNPAFAT
ISKVANSLGLRLSIKPLSRPEAGS

Nucleotide

• Length: 315 bp

>AT168224 NZ_CP075665:485962-486216 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: A0A1G8NTB1