Detailed information of TA system TA168221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 1332154..1333064
• Replicon: chromosome
• Accession: NZ_CP059082
• Organism: Halomonas titanicae strain SOB56

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: HZS52_RS06275
• Protein ID: WP_089691167.1
• Coordinates: 1332154..1332618 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A653Z8I6
• Locus tag: HZS52_RS06280
• Protein ID: WP_022521350.1
• Coordinates: 1332615..1333064 (-)
• Length: 150 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HZS52_RS06250	1327306..1328013	+	708	WP_009286159.1	urea ABC transporter ATP-binding subunit UrtE	-
HZS52_RS06255	1328111..1329031	-	921	WP_009286158.1	metal ABC transporter permease	-
HZS52_RS06260	1329028..1330134	-	1107	WP_009286157.1	metal ABC transporter permease	-
HZS52_RS06265	1330138..1330920	-	783	WP_089691170.1	ABC transporter ATP-binding protein	-
HZS52_RS06270	1330943..1331911	-	969	WP_089691169.1	zinc ABC transporter substrate-binding protein	-
HZS52_RS06275	1332154..1332618	-	465	WP_089691167.1	RES family NAD+ phosphorylase	Toxin
HZS52_RS06280	1332615..1333064	-	450	WP_022521350.1	DUF2384 domain-containing protein	Antitoxin
HZS52_RS06285	1333253..1334614	+	1362	WP_022521351.1	diguanylate cyclase	-
HZS52_RS06290	1334842..1335726	+	885	WP_159177683.1	urease accessory protein UreD	-
HZS52_RS06295	1335764..1336066	+	303	WP_022521353.1	urease subunit gamma	-
HZS52_RS06300	1336220..1336540	+	321	WP_190374333.1	urease subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (6-149)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-90)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 16850.17 Da
• Isoelectric Point: 4.1956

>T168221 WP_089691167.1 NZ_CP059082:c1332618-1332154 [Halomonas titanicae]
MSEVTAYRLVKRKFQETAFDGEGARLYGGRWNSPGNACVYVASYESLALLEIMVNLESYRLLNAYALLRLTLPADSILSI
GAEDLPENWQEAPAPAETAELGDGWLASGQSLALAVPSVVVPRELNYMLNPAHPLFDQIVATAEALPFQPDPRL

Nucleotide

• Length: 465 bp

>T168221 NZ_CP075663:4604540-4604938 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 150 a.a.
• Molecular weight: 16672.13 Da
• Isoelectric Point: 10.0368

>AT168221 WP_022521350.1 NZ_CP059082:c1333064-1332615 [Halomonas titanicae]
MDIHFTQYQPPKALASGFWQQIGLPARGRKLHELLHEGVEYRVYPQIAKVSGIEQKALARYVDIPSATLSRRAKSGRFKM
AESDRLYRFAELFKAALDLFEGDSEGARAWLLKPNAGLGGRRPVEMSATSAEYQTVLNLIGRLEHGVSV

Nucleotide

• Length: 450 bp

>AT168221 NZ_CP075663:4604313-4604540 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A653Z8I6