Detailed information of TA system TA168216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 3259112..3259700
• Replicon: chromosome
• Accession: NZ_CP059080
• Organism: Acinetobacter johnsonii strain E10B

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A2W5RB36
• Locus tag: H0S57_RS15840
• Protein ID: WP_039890600.1
• Coordinates: 3259413..3259700 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: H0S57_RS15835
• Protein ID: WP_126034950.1
• Coordinates: 3259112..3259426 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0S57_RS15805	3255058..3256005	+	948	Protein_3052	sensor domain-containing diguanylate cyclase	-
H0S57_RS15810	3256212..3256457	+	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
H0S57_RS15815	3256447..3256740	+	294	WP_119686978.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H0S57_RS15820	3256927..3257973	+	1047	WP_195728336.1	hypothetical protein	-
H0S57_RS15825	3257970..3258434	+	465	WP_126034954.1	hypothetical protein	-
H0S57_RS15830	3258421..3259047	+	627	WP_126034952.1	hypothetical protein	-
H0S57_RS15835	3259112..3259426	-	315	WP_126034950.1	BrnA antitoxin family protein	Antitoxin
H0S57_RS15840	3259413..3259700	-	288	WP_039890600.1	BrnT family toxin	Toxin
H0S57_RS15845	3260426..3261550	+	1125	WP_195728337.1	ABC transporter ATP-binding protein	-
H0S57_RS15850	3261572..3262588	+	1017	WP_005401415.1	extracellular solute-binding protein	-
H0S57_RS15855	3262670..3264298	+	1629	WP_151836186.1	iron ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	katA	3219938..3320368	100430

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11317.74 Da
• Isoelectric Point: 6.0889

>T168216 WP_039890600.1 NZ_CP059080:c3259700-3259413 [Acinetobacter johnsonii]
MEQYFEWDEAKNRKKQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNQYEHG

Nucleotide

• Length: 288 bp

>T168216 NZ_CP075663:3488877-3489152 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12233.90 Da
• Isoelectric Point: 10.0927

>AT168216 WP_126034950.1 NZ_CP059080:c3259426-3259112 [Acinetobacter johnsonii]
MSMVRYTRKELNENFSDKQDAEIKRLLAKGTVPDDQLDLSDIPEITDWSNAVRHGQFYRPVKQQTSVRLDADVLAWLKSQ
GKGYQTRMNKILREAMLNERKNHR

Nucleotide

• Length: 315 bp

>AT168216 NZ_CP075663:3489149-3489508 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A2W5RB36