Detailed information of TA system TA168215

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 3256212..3256740
• Replicon: chromosome
• Accession: NZ_CP059080
• Organism: Acinetobacter johnsonii strain E10B

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H0S57_RS15815
• Protein ID: WP_119686978.1
• Coordinates: 3256447..3256740 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9B5Q7
• Locus tag: H0S57_RS15810
• Protein ID: WP_000246756.1
• Coordinates: 3256212..3256457 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0S57_RS15795	3251946..3252710	+	765	WP_078426309.1	IS5 family transposase	-
H0S57_RS15800	3253356..3254876	+	1521	WP_151837270.1	catalase	-
H0S57_RS15805	3255058..3256005	+	948	Protein_3052	sensor domain-containing diguanylate cyclase	-
H0S57_RS15810	3256212..3256457	+	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H0S57_RS15815	3256447..3256740	+	294	WP_119686978.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H0S57_RS15820	3256927..3257973	+	1047	WP_195728336.1	hypothetical protein	-
H0S57_RS15825	3257970..3258434	+	465	WP_126034954.1	hypothetical protein	-
H0S57_RS15830	3258421..3259047	+	627	WP_126034952.1	hypothetical protein	-
H0S57_RS15835	3259112..3259426	-	315	WP_126034950.1	BrnA antitoxin family protein	-
H0S57_RS15840	3259413..3259700	-	288	WP_039890600.1	BrnT family toxin	-
H0S57_RS15845	3260426..3261550	+	1125	WP_195728337.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	katA	3219938..3320368	100430
	-	flank	IS/Tn	-	-	3251946..3252710	764

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-71)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11319.59 Da
• Isoelectric Point: 10.5382

>T168215 WP_119686978.1 NZ_CP059080:3256447-3256740 [Acinetobacter johnsonii]
MTYKLLRHKDFKAEWEKLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T168215 NZ_CP075663:c3022263-3021952 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9235.61 Da
• Isoelectric Point: 4.3142

>AT168215 WP_000246756.1 NZ_CP059080:3256212-3256457 [Acinetobacter johnsonii]
MATVNFRVDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT168215 NZ_CP075663:c3021951-3021661 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: N9B5Q7