Detailed information of TA system TA168201

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3013661..3014289
• Replicon: chromosome
• Accession: NZ_CP059066
• Organism: Koleobacter methoxysyntrophicus strain NRmbB1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H0A61_RS14810
• Protein ID: WP_206707858.1
• Coordinates: 3013897..3014289 (+)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: H0A61_RS14805
• Protein ID: WP_206707857.1
• Coordinates: 3013661..3013891 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0A61_RS14775	3009705..3010283	-	579	WP_206707851.1	thymidine kinase	-
H0A61_RS14780	3010351..3010533	-	183	WP_206707852.1	hypothetical protein	-
H0A61_RS14785	3010536..3011261	-	726	WP_206707853.1	host-nuclease inhibitor Gam family protein	-
H0A61_RS14790	3011350..3011580	-	231	WP_206707854.1	hypothetical protein	-
H0A61_RS14795	3011681..3011947	-	267	WP_206707855.1	hypothetical protein	-
H0A61_RS14800	3012072..3012992	-	921	WP_206707856.1	hypothetical protein	-
H0A61_RS14805	3013661..3013891	+	231	WP_206707857.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H0A61_RS14810	3013897..3014289	+	393	WP_206707858.1	PIN domain-containing protein	Toxin
H0A61_RS14815	3014436..3015302	+	867	WP_206707859.1	site-specific DNA-methyltransferase	-
H0A61_RS14820	3015299..3017116	+	1818	WP_206707860.1	N-6 DNA methylase	-
H0A61_RS14825	3017282..3018616	-	1335	WP_206707861.1	IS200/IS605 family accessory protein TnpB-related protein	-
H0A61_RS14830	3018582..3019010	-	429	WP_206707862.1	IS200/IS605 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2935016..3019010	83994

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-113)

Antitoxin

• SpoVT-AbrB domain (9-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14521.11 Da
• Isoelectric Point: 6.9045

>T168201 WP_206707858.1 NZ_CP059066:3013897-3014289 [Koleobacter methoxysyntrophicus]
MLDTNICIYIIKKKPVKVIERLKKLDIGDICISVITLAELCYGIEKSQNKERNRLALAAFLAPIEILPFSDEAAARYGEI
RAFLEKTGQIIGAYDLLIAAHAVSKNLILITNNVSEFGRVPCLSIENWTE

Nucleotide

• Length: 393 bp

>T168201 NZ_CP075654:c18675-18289 [Escherichia coli]
ATGAAATTCGTTAGTTCACAGGAAGTGATTGAGTTTCATGACCGCTTGATTTCGCGTGATGGTGGCGTGGCGGGTATGCC
AGAACCCGGTCGCGCTGACGCGATCATTCATCGTGTTTTGAACATGTATCATTATGAAGGTGTGACAGATATTATGGATC
TGGCTGCCGTGTATTTAGTTGCTATTGCTCGTGGTCACATTTTCAACGATGCAAATAAGCGAACAGCCTTGTTTGTTGCA
CAGGTTTTTTTAAAACGGAATGGTGTACATATTATTAGTTCACGTATTTCGTTTGATGAAATGCAAATTATCGCTTTGAA
TGCTGCAACAGGAGAATATAACTGGAAGATGGTTTCTGACCATCTTAAAGCCATTATTCTAAATTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8970.16 Da
• Isoelectric Point: 7.0125

>AT168201 WP_206707857.1 NZ_CP059066:3013661-3013891 [Koleobacter methoxysyntrophicus]
VDVAKIFENGRSQAVRLPKQYRFNDSEVFIKKVGDIVVLIPKESVWKSFEYGLKHFSEDFLNTRNQPEPEKRDDIQ

Nucleotide

• Length: 231 bp

>AT168201 NZ_CP075654:c18896-18672 [Escherichia coli]
ATGTTTACATGTAACTATAGCCATGCTCGCGATAACCTGGCCTCGTTGCTCGAGCTGGCGGTTGATGAAAATCAGCCAGT
TGAGATTCAGCGCCGTAAAGGTGGCGCAGCTGTACTGGTTGATAAAGACGCTTTTGAACGATACCGTAAAGCGGCTCTTG
ATGCTGAGTTTGCTGAGATCATGCAAAGTCATGGGACGGCCTATACGGAGCTTTCTAAGCGATGA