Detailed information of TA system TA168194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 9035..9566
• Replicon: plasmid pBS1142
• Accession: NZ_CP059054
• Organism: Pseudomonas putida strain BS3701

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: H0H12_RS29600
• Protein ID: WP_180690175.1
• Coordinates: 9285..9566 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: -
• Locus tag: H0H12_RS29595
• Protein ID: WP_180690176.1
• Coordinates: 9035..9298 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0H12_RS29565	4478..6139	-	1662	WP_180690178.1	DDE-type integrase/transposase/recombinase	-
H0H12_RS29570	6120..6695	-	576	WP_180690177.1	recombinase family protein	-
H0H12_RS29575	6824..7390	-	567	WP_003159185.1	recombinase family protein	-
H0H12_RS29580	7572..7823	+	252	WP_003108276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
H0H12_RS29585	7820..8215	+	396	WP_003159186.1	type II toxin-antitoxin system VapC family toxin	-
H0H12_RS29590	8221..8556	+	336	Protein_12	transposase	-
H0H12_RS29595	9035..9298	+	264	WP_180690176.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
H0H12_RS29600	9285..9566	+	282	WP_180690175.1	type II toxin-antitoxin system YafQ family toxin	Toxin
H0H12_RS29605	9603..9787	+	185	Protein_15	hypothetical protein	-
H0H12_RS29610	9787..10035	+	249	Protein_16	hypothetical protein	-
H0H12_RS29615	10125..11750	-	1626	WP_180690174.1	methyl-accepting chemotaxis protein	-
H0H12_RS29620	12053..12910	-	858	WP_180690173.1	phosphate/phosphite/phosphonate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..54501	54501

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Antitoxin

• Toxin YoeB (5-84)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 12066.38 Da
• Isoelectric Point: 12.7685

>T168194 WP_180690175.1 NZ_CP059054:9285-9566 [Pseudomonas putida]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKKKKKKKK

Nucleotide

• Length: 282 bp

>T168194 NZ_CP075652:4564936-4565187 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 11297.34 Da
• Isoelectric Point: 12.7395

>AT168194 WP_180690176.1 NZ_CP059054:9035-9298 [Pseudomonas putida]
KKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKKK
KKKKKKKK

Nucleotide

• Length: 264 bp

>AT168194 NZ_CP075652:4565184-4565438 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA