Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-DUF971
Location 12843..13444 Replicon plasmid pBS1141
Accession NZ_CP059053
Organism Pseudomonas putida strain BS3701

Toxin (Protein)


Gene name PumA Uniprot ID Q8VMN7
Locus tag H0H12_RS28960 Protein ID WP_011005862.1
Coordinates 13142..13444 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID Q8VMN8
Locus tag H0H12_RS28955 Protein ID WP_011005861.1
Coordinates 12843..13145 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0H12_RS28930 8108..9394 - 1287 WP_180690183.1 Y-family DNA polymerase -
H0H12_RS28935 9384..9815 - 432 WP_011005854.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
H0H12_RS28940 9953..10903 + 951 WP_062378615.1 non-homologous end-joining DNA ligase -
H0H12_RS28945 10934..11209 + 276 WP_011005856.1 hypothetical protein -
H0H12_RS28950 12578..12802 - 225 WP_011005860.1 hypothetical protein -
H0H12_RS28955 12843..13145 - 303 WP_011005861.1 putative addiction module antidote protein Antitoxin
H0H12_RS28960 13142..13444 - 303 WP_011005862.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H0H12_RS28965 13646..14425 + 780 WP_180690184.1 DUF4942 domain-containing protein -
H0H12_RS28970 14579..14947 + 369 WP_011005864.1 hypothetical protein -
H0H12_RS28975 14980..15297 + 318 WP_062378618.1 hypothetical protein -
H0H12_RS28980 15353..15571 + 219 WP_011005866.1 hypothetical protein -
H0H12_RS28985 15710..16729 + 1020 WP_062378625.1 hypothetical protein -
H0H12_RS28990 16872..17114 + 243 WP_011005868.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..107388 107388


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 14882.85 Da        Isoelectric Point: 5.5500

>T168191 WP_011005862.1 NZ_CP059053:c13444-13142 [Pseudomonas putida]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFF

Download         Length: 303 bp

>T168191 NZ_CP075652:3664205-3664612 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 14882.85 Da        Isoelectric Point: 5.5500

>AT168191 WP_011005861.1 NZ_CP059053:c13145-12843 [Pseudomonas putida]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFF

Download         Length: 303 bp

>AT168191 NZ_CP075652:3663958-3664224 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2K4KM11

Antitoxin

Source ID Structure
AlphaFold DB Q8VMN8

References