Detailed information of TA system TA168190

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4976784..4977567
• Replicon: chromosome
• Accession: NZ_CP059047
• Organism: Raoultella ornithinolytica strain DSM 16926

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: HYH04_RS23175
• Protein ID: WP_048225456.1
• Coordinates: 4976784..4977158 (-)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: HYH04_RS23180
• Protein ID: WP_048225453.1
• Coordinates: 4977208..4977567 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH04_RS23155	4972344..4973288	-	945	WP_180553472.1	hypothetical protein	-
HYH04_RS23160	4973288..4974265	-	978	WP_180553474.1	thymidylate synthase	-
HYH04_RS23165	4975256..4976245	+	990	WP_180553476.1	glycosyltransferase	-
HYH04_RS23170	4976384..4976704	+	321	WP_117074233.1	hypothetical protein	-
HYH04_RS23175	4976784..4977158	-	375	WP_048225456.1	TA system toxin CbtA family protein	Toxin
HYH04_RS23180	4977208..4977567	-	360	WP_048225453.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HYH04_RS23185	4977590..4977811	-	222	WP_032755299.1	DUF987 domain-containing protein	-
HYH04_RS23190	4977825..4978307	-	483	WP_180553478.1	DNA repair protein RadC	-
HYH04_RS23195	4978304..4978537	-	234	WP_010434188.1	hypothetical protein	-
HYH04_RS23200	4978548..4979027	-	480	WP_180553480.1	antirestriction protein	-
HYH04_RS23205	4979107..4979928	-	822	WP_180553482.1	DUF945 domain-containing protein	-
HYH04_RS23210	4980035..4980247	-	213	WP_180553484.1	hypothetical protein	-
HYH04_RS23215	4980334..4980693	-	360	WP_004136901.1	hypothetical protein	-
HYH04_RS23220	4980748..4981212	-	465	WP_004114621.1	hypothetical protein	-
HYH04_RS23225	4981490..4982347	-	858	WP_163387552.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4970524..5003231	32707

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (8-112)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13974.16 Da
• Isoelectric Point: 10.3161

>T168190 WP_048225456.1 NZ_CP059047:c4977158-4976784 [Raoultella ornithinolytica]
MQIHSLSLQRAASSRLSSVEIWRKLLKYLLKQHYGLMLNDTPFGNDEVIQKHIDAGISLCDAVNFIVEKYDLVRTDRHGF
SVKEQSPFIGSIDMLRARKATGLMTRKGYKTVTDITGGRFSGGK

Nucleotide

• Length: 375 bp

>T168190 NZ_CP075652:c3531009-3530635 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCGCGTCCGTCTCCTGTTGAAATCTGGCAGACACTGCTGTC
CCGCCTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCTCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCCCGGTTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCGGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13450.58 Da
• Isoelectric Point: 7.3729

>AT168190 WP_048225453.1 NZ_CP059047:c4977567-4977208 [Raoultella ornithinolytica]
MQKATRVINHNITEPWWGLRRNITPCFGARLVHEGNHLHYLADRASIAGTFNDADLRHLDQVFPVLMKQMELMLASSELT
PRIQRCITLHVKGLICEADTLGSCGYLYIVIYPASATTE

Nucleotide

• Length: 360 bp

>AT168190 NZ_CP075652:c3531467-3531099 [Escherichia coli]
GTGTCAGACACGCACCATGAGACAAATTATCCCGACGACTATAACAACCGCCCCTGGTGGGGGCTGCCCTGCACCATAAC
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGTATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAAGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATACTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGACTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA