Detailed information of TA system TA168188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3823015..3823562
• Replicon: chromosome
• Accession: NZ_CP059047
• Organism: Raoultella ornithinolytica strain DSM 16926

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A808XUV0
• Locus tag: HYH04_RS17645
• Protein ID: WP_048025274.1
• Coordinates: 3823015..3823323 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: HYH04_RS17650
• Protein ID: WP_088883552.1
• Coordinates: 3823326..3823562 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH04_RS17615	3818203..3818943	+	741	WP_015585491.1	4-oxalmesaconate hydratase	-
HYH04_RS17620	3818940..3819653	+	714	WP_015585492.1	4-carboxy-4-hydroxy-2-oxoadipate aldolase/oxaloacetate decarboxylase	-
HYH04_RS17625	3819658..3820749	+	1092	WP_015585493.1	4-oxalomesaconate tautomerase	-
HYH04_RS17630	3820746..3821612	+	867	WP_015585494.1	NAD(P)-dependent oxidoreductase	-
HYH04_RS17635	3821613..3821888	-	276	WP_167324217.1	hypothetical protein	-
HYH04_RS17640	3821997..3822899	-	903	WP_015585496.1	EamA family transporter	-
HYH04_RS17645	3823015..3823323	-	309	WP_048025274.1	CcdB family protein	Toxin
HYH04_RS17650	3823326..3823562	-	237	WP_088883552.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
HYH04_RS17655	3823668..3825101	-	1434	WP_069476354.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
HYH04_RS17660	3825126..3826028	-	903	WP_064359684.1	N-acetylmuramic acid 6-phosphate etherase	-
HYH04_RS17665	3826193..3827356	-	1164	WP_004869055.1	multidrug effflux MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11778.62 Da
• Isoelectric Point: 6.7156

>T168188 WP_048025274.1 NZ_CP059047:c3823323-3823015 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLFDGI

Nucleotide

• Length: 309 bp

>T168188 NZ_CP075652:3019966-3020196 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8913.05 Da
• Isoelectric Point: 5.6579

>AT168188 WP_088883552.1 NZ_CP059047:c3823562-3823326 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTQDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT168188 NZ_CP075652:3020193-3020552 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A808XUV0