Detailed information of TA system TA168187

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3044085..3044664
• Replicon: chromosome
• Accession: NZ_CP059047
• Organism: Raoultella ornithinolytica strain DSM 16926

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: HYH04_RS14065
• Protein ID: WP_015585223.1
• Coordinates: 3044377..3044664 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: HYH04_RS14060
• Protein ID: WP_015585222.1
• Coordinates: 3044085..3044390 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH04_RS14035	3039293..3040357	-	1065	WP_004857527.1	DUF2955 domain-containing protein	-
HYH04_RS14040	3040347..3041414	-	1068	WP_004857525.1	HlyD family secretion protein	-
HYH04_RS14045	3041421..3041885	-	465	WP_004857523.1	MarR family transcriptional regulator	-
HYH04_RS14050	3042176..3042340	-	165	WP_004857521.1	DUF1127 domain-containing protein	-
HYH04_RS14055	3042518..3043930	+	1413	WP_015585221.1	PLP-dependent aminotransferase family protein	-
HYH04_RS14060	3044085..3044390	-	306	WP_015585222.1	BrnA antitoxin family protein	Antitoxin
HYH04_RS14065	3044377..3044664	-	288	WP_015585223.1	BrnT family toxin	Toxin
HYH04_RS14070	3044781..3045200	-	420	WP_015585224.1	FosA family fosfomycin resistance glutathione transferase	-
HYH04_RS14075	3045194..3046102	-	909	WP_015585225.1	LysR family transcriptional regulator	-
HYH04_RS14080	3046190..3046972	+	783	WP_015585226.1	NAD(P)H-dependent oxidoreductase	-
HYH04_RS14085	3047120..3047698	+	579	WP_004857495.1	TetR/AcrR family transcriptional regulator	-
HYH04_RS14090	3047842..3048654	+	813	WP_004857492.1	winged helix-turn-helix domain-containing protein	-
HYH04_RS14095	3048651..3049169	+	519	WP_015585229.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (40-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11067.49 Da
• Isoelectric Point: 7.0747

>T168187 WP_015585223.1 NZ_CP059047:c3044664-3044377 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRQDRYENGEHRWQTIGLVHGIIVILVVHSVRFESGAEVIRIIS
ARKADGKERSRYEHS

Nucleotide

• Length: 288 bp

>T168187 NZ_CP075652:c2725124-2724747 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGTATCCGGAGGCAAAACGATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11526.01 Da
• Isoelectric Point: 9.7545

>AT168187 WP_015585222.1 NZ_CP059047:c3044390-3044085 [Raoultella ornithinolytica]
MSTVKYKRDNPAGLSAQREDELRALANKADGEIDYSDIPASGDEQWPDAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIREQNKKS

Nucleotide

• Length: 306 bp

>AT168187 NZ_CP075652:c2725581-2725213 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA