Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2473897..2474516 Replicon chromosome
Accession NZ_CP059047
Organism Raoultella ornithinolytica strain DSM 16926

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag HYH04_RS11395 Protein ID WP_004858783.1
Coordinates 2474298..2474516 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag HYH04_RS11390 Protein ID WP_004858785.1
Coordinates 2473897..2474271 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HYH04_RS11380 2469008..2470201 + 1194 WP_004858789.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
HYH04_RS11385 2470224..2473370 + 3147 WP_004858787.1 multidrug efflux RND transporter permease subunit -
HYH04_RS11390 2473897..2474271 + 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
HYH04_RS11395 2474298..2474516 + 219 WP_004858783.1 hemolysin expression modulator Hha Toxin
HYH04_RS11400 2474668..2475234 + 567 WP_004858781.1 maltose O-acetyltransferase -
HYH04_RS11405 2475334..2475804 + 471 WP_128318549.1 YlaC family protein -
HYH04_RS11410 2475779..2477230 - 1452 WP_069476198.1 PLP-dependent aminotransferase family protein -
HYH04_RS11415 2477334..2478044 + 711 WP_004858778.1 GNAT family N-acetyltransferase -
HYH04_RS11420 2478041..2478181 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
HYH04_RS11425 2478184..2478444 - 261 WP_004858777.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T168186 WP_004858783.1 NZ_CP059047:2474298-2474516 [Raoultella ornithinolytica]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T168186 NZ_CP075652:c2478096-2477785 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT168186 WP_004858785.1 NZ_CP059047:2473897-2474271 [Raoultella ornithinolytica]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT168186 NZ_CP075652:c2477784-2477494 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References