Detailed information of TA system TA168185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 1100208..1101811
• Replicon: chromosome
• Accession: NZ_CP059047
• Organism: Raoultella ornithinolytica strain DSM 16926

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: HYH04_RS05100
• Protein ID: WP_064359332.1
• Coordinates: 1100208..1101539 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A808YAX5
• Locus tag: HYH04_RS05105
• Protein ID: WP_032717448.1
• Coordinates: 1101539..1101811 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH04_RS05085	1096082..1096996	-	915	WP_088883967.1	branched-chain amino acid ABC transporter permease LivH	-
HYH04_RS05090	1097096..1098154	-	1059	WP_162180097.1	branched-chain amino acid ABC transporter substrate-binding protein	-
HYH04_RS05095	1098860..1100275	+	1416	WP_099144431.1	glycoside hydrolase family 10 protein	-
HYH04_RS05100	1100208..1101539	-	1332	WP_064359332.1	type II toxin-antitoxin system HipA family toxin	Toxin
HYH04_RS05105	1101539..1101811	-	273	WP_032717448.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
HYH04_RS05110	1102015..1102569	-	555	WP_015584337.1	RNA polymerase sigma factor	-
HYH04_RS05115	1102575..1103000	-	426	WP_097357846.1	hypothetical protein	-
HYH04_RS05120	1103133..1104086	-	954	WP_180553881.1	hypothetical protein	-
HYH04_RS05125	1104300..1104932	-	633	WP_048023555.1	class I SAM-dependent methyltransferase	-
HYH04_RS05130	1104952..1106193	-	1242	WP_180553882.1	sensor domain-containing diguanylate cyclase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (7-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 49355.71 Da
• Isoelectric Point: 6.7012

>T168185 WP_064359332.1 NZ_CP059047:c1101539-1100208 [Raoultella ornithinolytica]
MATLVTWMNNERVGELIKQANGAHLFRYEEAWMRNPRARPLSLSLPLQYGNITDEAVFNYFDNLLPDSPRVRDRIVKRYQ
ARSKQPFDLLAEIGRDSVGAVTLLPPGENVPRGALSWEPLDNHALDALLTAYQSDIPLGMVNEHDDFRISVAGAQEKTAL
LKIGEQWCIPTGTTPTTHIIKLPIGEIKQPDAVLDLSESVDNEYLCLALARALGFAVPPASIIQTASIRALAVERFDRRW
AQEKTVLLRLPQEDLCQAFGIPSSIKYESDGGPGIRAIMAFLVGSSEALEDRYNFMKFMVFQWLIGATDGHAKNFSIFLQ
PGGSYRLTPFYDIISAFPLLGGKGLHLSDLKLSMSLKASKGRKTEIQTLYPRHFLATAKEVGFAQQQMLEILRYFVDNVP
RAVEAVKGTLPPDFSQPVYEAITDRLLWVHSRLQNAMAASPSG

Nucleotide

• Length: 1332 bp

>T168185 NZ_CP075652:c2192704-2192531 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10365.71 Da
• Isoelectric Point: 5.7971

>AT168185 WP_032717448.1 NZ_CP059047:c1101811-1101539 [Raoultella ornithinolytica]
MSPSPTIYSPVQLANYLKLVRQKNQWTQSELAQRMGIKQATVSNFENHPEKTTLTTVFKILQSLELSMAICEKNAPDTDD
NRDNPRDLEW

Nucleotide

• Length: 273 bp

>AT168185 NZ_CP075652:c2193028-2192732 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A808YAX5