Detailed information of TA system TA168184

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 1089771..1090522
• Replicon: chromosome
• Accession: NZ_CP059047
• Organism: Raoultella ornithinolytica strain DSM 16926

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: HYH04_RS05050
• Protein ID: WP_044351088.1
• Coordinates: 1090040..1090522 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A808Y4Y8
• Locus tag: HYH04_RS05045
• Protein ID: WP_015584326.1
• Coordinates: 1089771..1090049 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HYH04_RS05030	1084977..1086344	+	1368	Protein_987	KUP/HAK/KT family potassium transporter	-
HYH04_RS05035	1086560..1087057	+	498	WP_048023692.1	hypothetical protein	-
HYH04_RS05040	1087215..1089140	-	1926	WP_180553877.1	C1 family peptidase	-
HYH04_RS05045	1089771..1090049	+	279	WP_015584326.1	DUF1778 domain-containing protein	Antitoxin
HYH04_RS05050	1090040..1090522	+	483	WP_044351088.1	GNAT family N-acetyltransferase	Toxin
HYH04_RS05055	1090707..1091057	+	351	WP_044351090.1	hypothetical protein	-
HYH04_RS05060	1091167..1091325	-	159	WP_004861965.1	hypothetical protein	-
HYH04_RS05065	1092063..1092938	-	876	WP_180553879.1	NAD(P)-dependent oxidoreductase	-
HYH04_RS05070	1093200..1093916	-	717	WP_004861956.1	ABC transporter ATP-binding protein	-
HYH04_RS05075	1093913..1094788	-	876	WP_015584331.1	ATP-binding cassette domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17612.48 Da
• Isoelectric Point: 9.7381

>T168184 WP_044351088.1 NZ_CP059047:1090040-1090522 [Raoultella ornithinolytica]
MGMRPPEPLSAGHNIAEFCCQDQVLNEWLKKKALKNHSTGISRVFVVCAENTKRVIAYYSLSSGSVHRNTVPGAYRRNAP
EAVPVIVLGRLAVDAAWAGKGLGAALLKDAIYRTEHIAVQVGVRALLVHALNDEVREFYTRFGFEPSIINTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T168184 NZ_CP075652:c2088524-2088174 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10714.30 Da
• Isoelectric Point: 4.6722

>AT168184 WP_015584326.1 NZ_CP059047:1089771-1090049 [Raoultella ornithinolytica]
MKPDVREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFASFMEELDAPMTAERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT168184 NZ_CP075652:c2088769-2088518 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A808Y4Y8