168142

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) rnlAB/RnlA-RnlB
Location 3200732..3202169 Replicon chromosome
Accession NZ_CP059043
Organism Escherichia coli strain SY3626C1

Toxin (Protein)


Gene name rnlA Uniprot ID P52129
Locus tag HZ987_RS15525 Protein ID WP_000155570.1
Coordinates 3200732..3201805 (+) Length 358 a.a.

Antitoxin (Protein)


Gene name rnlB Uniprot ID P52130
Locus tag HZ987_RS15530 Protein ID WP_000461704.1
Coordinates 3201798..3202169 (+) Length 124 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZ987_RS15510 3196165..3198354 - 2190 WP_000136402.1 DEAD/DEAH box helicase -
HZ987_RS15515 3198351..3199967 - 1617 WP_000445380.1 anti-bacteriophage protein AbpA -
HZ987_RS15520 3200327..3200590 - 264 WP_000502050.1 DUF1524 domain-containing protein -
HZ987_RS15525 3200732..3201805 + 1074 WP_000155570.1 type II toxin-antitoxin system toxin mRNA endoribonuclease RnlA Toxin
HZ987_RS15530 3201798..3202169 + 372 WP_000461704.1 type II toxin-antitoxin system antitoxin RnlB Antitoxin
HZ987_RS15535 3202524..3203387 + 864 WP_000787597.1 GTPase family protein -
HZ987_RS15540 3203479..3204300 + 822 WP_000197391.1 DUF945 domain-containing protein -
HZ987_RS15545 3204517..3205218 + 702 WP_000189409.1 WYL domain-containing protein -
HZ987_RS15550 3205259..3205495 + 237 WP_001144029.1 hypothetical protein -
HZ987_RS15555 3205495..3205938 + 444 WP_000824222.1 hypothetical protein -
HZ987_RS15560 3205962..3206429 + 468 WP_000702495.1 hypothetical protein -
HZ987_RS15565 3206654..3206968 - 315 WP_000065803.1 arsenate reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain

(98-183)

Antitoxin

Antitoxin RnlB/LsoB

(14-111)


Sequences

Toxin        


Download         Length: 358 a.a.        Molecular weight: 40061.13 Da        Isoelectric Point: 6.8293

>T168142 WP_000155570.1 NZ_CP059043:3200732-3201805 [Escherichia coli]
MTIRSYKNLNLVRANIETESRQFIENKNYSIQSIGPMPGSRAGLRVVFTRPGVNLATVDIFYNGDGSTTIQYLTGANRSL
GQELADHLFETINPAEFEQVNMVLQGFVETSVLPVLELSADESHIEFREHSRNAHTVVWKIISTSYQDELTVSLHITTGK
LQIQGRPLSCYRVFTFNLAALLDLQGLEKVLIRQEDGKANIVQQEVARTYLQTVMADAYPHLHVTAEKLLVSGLCVKLAA
PDLPDYCMLLYPELRTIEGVLKSKMSGLGMPVQQPAGFGTYFDKPAAHYILKPQFAATLRPEQINIISTAYTFFNVERHS
LFHMETVVDASRMISDMARLMGKATRAWGIIKDLYIV

Download         Length: 1074 bp

>T168142 NZ_CP075642:5954-6208 [Escherichia coli]
ATGAGAAAAAACAAAGGACGATTGACTTATTATCTTGAAATAAGTGATAAAAAATATCATTTTGTAAAAAAAATAAGCAG
CTATTCAAAGGAGTATACAGAAGGAAAAACTAAAACAACAAAGAGAACGTTAAGTGAACTGGTTTTTAATGAAAGCGAGG
TTGGGGCAATAGATTTTACAAAAAATGGTTTAAGACCTGTTGATAAGAATATTCTCTTAACTATGATGAAAGAATGTAAG
GAGAGTGATACATGA

Antitoxin


Download         Length: 124 a.a.        Molecular weight: 13664.69 Da        Isoelectric Point: 4.2391

>AT168142 WP_000461704.1 NZ_CP059043:3201798-3202169 [Escherichia coli]
MFEITGINVSGALKAVVMATGFENPLSSVNEIETKLSALLGSETTGEILFDLLCANGPEWNRFVTLEMKYGRIMLDTAKI
IDEQDVPTHILSKLTFTLRNHPEYLEASVLSPDDVRQVLSMDF

Download         Length: 372 bp

>AT168142 NZ_CP075642:6205-6441 [Escherichia coli]
ATGACCATTGAAAATACACCGGAAAACATAAAGAAATTACGTAAAAAAATAGGTCTTACTCAGACAGAGTGCGGTGAAAT
TTTTGGTGTAGGTTTAAGTACATGGCAGAAAAAGGAAGCAAAAACCCACAACCAGTTAAATTTGTCCAAAGGTGAATTTG
AGTACCTGTTATTGCTTGCAGGGGAACACCCTGATTATGTGCTTTGTAAAAGGAATTCTGATTCTGGAAATAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6Y2R
PDB 7AEX
PDB 6Y2Q
PDB 4I8O
PDB 6Y2P
PDB 6Y2S

Antitoxin

Source ID Structure
PDB 6Y2P
AlphaFold DB A0A373F4G4

References