Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/upstrm_HI1419-dnstrm_HI1420
Location 462564..463138 Replicon chromosome
Accession NZ_CP059035
Organism Pasteurella multocida subsp. multocida strain HN141014

Toxin (Protein)


Gene name PumA Uniprot ID -
Locus tag A0R66_RS02185 Protein ID WP_061406134.1
Coordinates 462564..462848 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID -
Locus tag A0R66_RS02190 Protein ID WP_061406133.1
Coordinates 462845..463138 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A0R66_RS02165 (A0R66_002155) 458357..459946 - 1590 WP_081106270.1 AAA family ATPase -
A0R66_RS02170 (A0R66_002160) 460231..460722 - 492 WP_256447825.1 NERD domain-containing protein -
A0R66_RS02175 (A0R66_002165) 460950..461285 + 336 WP_223848039.1 DUF3418 domain-containing protein -
A0R66_RS02185 (A0R66_002175) 462564..462848 + 285 WP_061406134.1 hypothetical protein Toxin
A0R66_RS02190 (A0R66_002180) 462845..463138 + 294 WP_061406133.1 putative addiction module antidote protein Antitoxin
A0R66_RS02195 (A0R66_002185) 463541..464218 - 678 WP_005726756.1 3-keto-L-gulonate-6-phosphate decarboxylase UlaD -
A0R66_RS02200 (A0R66_002190) 464236..464703 - 468 WP_064661066.1 PTS sugar transporter subunit IIA -
A0R66_RS02205 (A0R66_002195) 464748..466538 - 1791 WP_061406132.1 PTS ascorbate-specific subunit IIBC -
A0R66_RS02210 (A0R66_002200) 466888..467979 + 1092 WP_005751571.1 L-ascorbate 6-phosphate lactonase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(4-87)

Antitoxin

Uncharacterized protein HI1420

(7-92)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10667.40 Da        Isoelectric Point: 10.2297

>T168099 WP_061406134.1 NZ_CP059035:462564-462848 [Pasteurella multocida subsp. multocida]
VGRLKEVKILSAKSAVLARINKAMNGNFGDHKSIGNGLYEMRIIKGSGYRVYYGQYREVTYLLICGGDKSTQKSDIVKAR
ELWKEIKQQEEVKV

Download         Length: 285 bp

>T168099 NZ_CP075632:c3283169-3282858 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10947.88 Da        Isoelectric Point: 8.9149

>AT168099 WP_061406133.1 NZ_CP059035:462845-463138 [Pasteurella multocida subsp. multocida]
MSKKKIKLKLFDIAEHLDSEEMIAAYLSEVLETCDTNEFISALSDVVRAKGMTELAAKTGLRRESLYKTLSYGSKPRFDT
IMKIMHSLGIKLVPTHI

Download         Length: 294 bp

>AT168099 NZ_CP075632:c3282857-3282567 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References