Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 63136..63661 Replicon plasmid p2D-3
Accession NZ_CP059005
Organism Escherichia coli strain 2D

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag H0E72_RS25530 Protein ID WP_001159868.1
Coordinates 63136..63441 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H0E72_RS25535 Protein ID WP_000813634.1
Coordinates 63443..63661 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0E72_RS25515 59046..60212 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
H0E72_RS25520 60800..61555 - 756 WP_000852146.1 replication initiation protein RepE -
H0E72_RS25525 62329..63135 - 807 WP_000016982.1 site-specific integrase -
H0E72_RS25530 63136..63441 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
H0E72_RS25535 63443..63661 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H0E72_RS25540 64369..65364 + 996 WP_000246635.1 hypothetical protein -
H0E72_RS25545 65368..66300 + 933 WP_000991831.1 hypothetical protein -
H0E72_RS25550 66593..66778 + 186 WP_001586223.1 hypothetical protein -
H0E72_RS25555 66779..67681 + 903 WP_024187432.1 hypothetical protein -
H0E72_RS25560 67683..68552 + 870 WP_001586225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(B) / blaTEM-1B / aac(3)-IIa - 1..79323 79323


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T168098 WP_001159868.1 NZ_CP059005:c63441-63136 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T168098 NZ_CP075632:c3006777-3006604 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT168098 WP_000813634.1 NZ_CP059005:c63661-63443 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT168098 NZ_CP075632:c3007101-3006805 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References