Detailed information of TA system TA168097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 5252..5811
• Replicon: plasmid p2D-3
• Accession: NZ_CP059005
• Organism: Escherichia coli strain 2D

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: H0E72_RS25195
• Protein ID: WP_000421272.1
• Coordinates: 5252..5527 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: H0E72_RS25200
• Protein ID: WP_001387467.1
• Coordinates: 5527..5811 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0E72_RS25160	1301..2041	-	741	WP_001586233.1	tyrosine-type recombinase/integrase	-
H0E72_RS25165	2162..2344	-	183	WP_042004909.1	hypothetical protein	-
H0E72_RS25170	2411..2572	-	162	WP_001440647.1	type II toxin-antitoxin system RelE/ParE family toxin	-
H0E72_RS25175	2688..2956	-	269	Protein_4	type II toxin-antitoxin system ParD family antitoxin	-
H0E72_RS25180	3488..4657	+	1170	WP_001072355.1	hypothetical protein	-
H0E72_RS25185	4690..4818	+	129	Protein_6	IS481 family transposase	-
H0E72_RS25190	4853..5146	-	294	WP_001105060.1	hypothetical protein	-
H0E72_RS25195	5252..5527	-	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H0E72_RS25200	5527..5811	-	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
H0E72_RS25205	6416..7168	+	753	WP_000562172.1	IclR family transcriptional regulator	-
H0E72_RS25210	7214..8179	-	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
H0E72_RS25215	8212..8592	-	381	WP_023146450.1	Rid family detoxifying hydrolase	-
H0E72_RS25220	8617..9507	-	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
H0E72_RS25225	9908..10348	+	441	Protein_14	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / blaTEM-1B / aac(3)-IIa	-	1..79323	79323

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T168097 WP_000421272.1 NZ_CP059005:c5527-5252 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T168097 NZ_CP075632:2994188-2994565 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGCATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTACGAACTGACCAGCCAGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAATGGTAAATAACATTACCCAGGGTAAGCATCCGGAGGCGCAACAATGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT168097 WP_001387467.1 NZ_CP059005:c5811-5527 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT168097 NZ_CP075632:2993767-2994141 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAA
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGCGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGACATCAGCATTCCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGACTGTTTATCCGACACCGGCAACCGCAACCACCTCATAA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880