Detailed information of TA system TA168096

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1778..2337
• Replicon: plasmid p2D-CTX-M-14
• Accession: NZ_CP059004
• Organism: Escherichia coli strain 2D

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1PMX5
• Locus tag: H0E72_RS24610
• Protein ID: WP_000421257.1
• Coordinates: 2062..2337 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U9Y625
• Locus tag: H0E72_RS24605
• Protein ID: WP_001178087.1
• Coordinates: 1778..2062 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0E72_RS24600	1..873	+	873	WP_000729438.1	incFII family plasmid replication initiator RepA	-
H0E72_RS24605	1778..2062	+	285	WP_001178087.1	ribbon-helix-helix domain-containing protein	Antitoxin
H0E72_RS24610	2062..2337	+	276	WP_000421257.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H0E72_RS24615	2502..2783	+	282	WP_001105066.1	hypothetical protein	-
H0E72_RS24620	2974..3240	-	267	WP_000627831.1	hypothetical protein	-
H0E72_RS24625	3378..3980	-	603	WP_180555550.1	proQ/FINO family protein	-
H0E72_RS24630	4156..5208	-	1053	WP_000655519.1	hypothetical protein	-
H0E72_RS24635	5421..6026	-	606	WP_180555551.1	hypothetical protein	-
H0E72_RS24640	6026..6280	-	255	WP_000343106.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCTX-M-14	-	1..96499	96499

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 9.9324

>T168096 WP_000421257.1 NZ_CP059004:2062-2337 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T168096 NZ_CP075632:c2835866-2835516 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10488.92 Da
• Isoelectric Point: 5.6558

>AT168096 WP_001178087.1 NZ_CP059004:1778-2062 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT168096 NZ_CP075632:c2836111-2835860 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: S1PMX5

Antitoxin

• AlphaFold DB: U9Y625