Detailed information of TA system TA168092

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3969603..3970435
• Replicon: chromosome
• Accession: NZ_CP059003
• Organism: Escherichia coli strain 2D

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1PD64
• Locus tag: H0E72_RS19070
• Protein ID: WP_000854753.1
• Coordinates: 3970061..3970435 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NQ68
• Locus tag: H0E72_RS19065
• Protein ID: WP_001540478.1
• Coordinates: 3969603..3969971 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0E72_RS19030	3965436..3966116	+	681	WP_001282919.1	WYL domain-containing protein	-
H0E72_RS19035	3966264..3966941	+	678	WP_001097301.1	hypothetical protein	-
H0E72_RS19040	3966947..3967180	+	234	WP_001278283.1	DUF905 family protein	-
H0E72_RS19045	3967270..3968088	+	819	WP_001234682.1	DUF945 domain-containing protein	-
H0E72_RS19050	3968179..3968664	+	486	WP_000214398.1	antirestriction protein	-
H0E72_RS19055	3968680..3969156	+	477	WP_001313574.1	RadC family protein	-
H0E72_RS19060	3969219..3969440	+	222	WP_000692311.1	DUF987 domain-containing protein	-
H0E72_RS19065	3969603..3969971	+	369	WP_001540478.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H0E72_RS19070	3970061..3970435	+	375	WP_000854753.1	TA system toxin CbtA family protein	Toxin
H0E72_RS19075	3970432..3970920	+	489	WP_000777545.1	hypothetical protein	-
H0E72_RS19080	3970932..3971129	+	198	WP_000839281.1	DUF957 domain-containing protein	-
H0E72_RS19085	3971226..3971795	+	570	WP_001560692.1	DUF4942 domain-containing protein	-
H0E72_RS19090	3972153..3972308	+	156	WP_000729638.1	hypothetical protein	-
H0E72_RS19095	3973119..3974102	+	984	WP_024946586.1	KpsF/GutQ family sugar-phosphate isomerase	-
H0E72_RS19100	3974174..3975322	+	1149	WP_000905920.1	capsule polysaccharide export inner-membrane protein KpsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3972153..3972308	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 14005.00 Da
• Isoelectric Point: 8.2905

>T168092 WP_000854753.1 NZ_CP059003:3970061-3970435 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Nucleotide

• Length: 375 bp

>T168092 NZ_CP075632:c1478453-1478205 [Escherichia coli]
ATGGGGAAAGCAGATAAGCTACTGGAAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAGCTGGTCGTTTTGTT
TTCCTCTCTGGGATATGTCAAAAAGGAAATGCAGGGCTCAAGAGTGCGGTTTTTCAATGCTGAAATCAACCACACCATAT
TAATGCATCGCCCACATCCAGAAAGTTATATTAAAGGTGGAACGCTGAAAGCGATTAAACAGAATCTGAAAGAGGCTGGG
GTATTATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13652.53 Da
• Isoelectric Point: 7.8398

>AT168092 WP_001540478.1 NZ_CP059003:3969603-3969971 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT168092 NZ_CP075632:c1478208-1477864 [Escherichia coli]
ATGAAGCATCTAAAATATAAAGGATATTTAGGTACAGTAGAACCAGATTTTGAAAATAATATCCTGTACGGAAAACTGGC
GTTTATTCGAGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAGACATCGGTTGAGCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAGCCTGATACCCCGTTTAAAGGTGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCACTGAACGCCTTTGTTAATAAGGCGCTGGAAAAAGA
AGTCAACCATCACACAGGGGCTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9LVU0

Antitoxin

• AlphaFold DB: S1NQ68