Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3834536..3835190 Replicon chromosome
Accession NZ_CP059003
Organism Escherichia coli strain 2D

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag H0E72_RS18355 Protein ID WP_000244772.1
Coordinates 3834536..3834943 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag H0E72_RS18360 Protein ID WP_000354046.1
Coordinates 3834924..3835190 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0E72_RS18335 3830493..3832226 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
H0E72_RS18340 3832232..3832942 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
H0E72_RS18345 3832967..3833863 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
H0E72_RS18350 3833975..3834496 + 522 WP_001055874.1 flavodoxin FldB -
H0E72_RS18355 3834536..3834943 - 408 WP_000244772.1 protein YgfX Toxin
H0E72_RS18360 3834924..3835190 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
H0E72_RS18365 3835433..3836413 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
H0E72_RS18370 3836490..3837149 - 660 WP_000250274.1 hemolysin III family protein -
H0E72_RS18375 3837313..3837624 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
H0E72_RS18380 3837669..3839102 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
H0E72_RS18385 3839159..3839902 - 744 WP_075591686.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T168091 WP_000244772.1 NZ_CP059003:c3834943-3834536 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T168091 NZ_CP075632:1208649-1208756 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT168091 WP_000354046.1 NZ_CP059003:c3835190-3834924 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT168091 NZ_CP075632:c1208599-1208536 [Escherichia coli]
TCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References