Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2436501..2437139 Replicon chromosome
Accession NZ_CP059003
Organism Escherichia coli strain 2D

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag H0E72_RS11800 Protein ID WP_000813794.1
Coordinates 2436963..2437139 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag H0E72_RS11795 Protein ID WP_001270286.1
Coordinates 2436501..2436917 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0E72_RS11775 2431653..2432594 - 942 WP_001251303.1 ABC transporter permease -
H0E72_RS11780 2432595..2433608 - 1014 WP_000220391.1 ABC transporter ATP-binding protein -
H0E72_RS11785 2433626..2434771 - 1146 WP_000034364.1 ABC transporter substrate-binding protein -
H0E72_RS11790 2435016..2436422 - 1407 WP_000760611.1 PLP-dependent aminotransferase family protein -
H0E72_RS11795 2436501..2436917 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
H0E72_RS11800 2436963..2437139 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
H0E72_RS11805 2437361..2437591 + 231 WP_000491567.1 DUF2554 family protein -
H0E72_RS11810 2437683..2439644 - 1962 WP_012311648.1 23S rRNA 5-hydroxycytidine C2501 synthase -
H0E72_RS11815 2439717..2440253 - 537 WP_000429154.1 DNA-binding transcriptional regulator SutR -
H0E72_RS11820 2440306..2441226 + 921 Protein_2308 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2441241..2442569 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T168088 WP_000813794.1 NZ_CP059003:c2437139-2436963 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T168088 NZ_CP075632:c931221-931045 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT168088 WP_001270286.1 NZ_CP059003:c2436917-2436501 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT168088 NZ_CP075632:c930999-930583 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References