Detailed information of TA system TA168078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1478887..1479592
• Replicon: chromosome
• Accession: NZ_CP059003
• Organism: Escherichia coli strain 2D

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: H0E72_RS06920
• Protein ID: WP_000539521.1
• Coordinates: 1479206..1479592 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H0E72_RS06915
• Protein ID: WP_001280945.1
• Coordinates: 1478887..1479216 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0E72_RS06900	1474045..1474956	+	912	WP_001236058.1	glutathione ABC transporter permease GsiD	-
H0E72_RS06905	1475133..1477481	+	2349	WP_000950337.1	EAL domain-containing protein	-
H0E72_RS06910	1477489..1478817	+	1329	WP_000086869.1	GGDEF domain-containing protein	-
H0E72_RS06915	1478887..1479216	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
H0E72_RS06920	1479206..1479592	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H0E72_RS06925	1479818..1481143	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
H0E72_RS06930	1481356..1481739	+	384	WP_000497137.1	biofilm formation regulator BssR	-
H0E72_RS06935	1481850..1482965	+	1116	WP_000555050.1	aldose sugar dehydrogenase YliI	-
H0E72_RS06940	1482962..1483588	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T168078 WP_000539521.1 NZ_CP059003:c1479592-1479206 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T168078 NZ_CP075627:4138267-4138731 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT168078 WP_001280945.1 NZ_CP059003:c1479216-1478887 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT168078 NZ_CP075627:4137932-4138267 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6