Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1124243..1124861 Replicon chromosome
Accession NZ_CP059003
Organism Escherichia coli strain 2D

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H0E72_RS05220 Protein ID WP_001291435.1
Coordinates 1124243..1124461 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H0E72_RS05225 Protein ID WP_000344800.1
Coordinates 1124487..1124861 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0E72_RS05185 1119533..1120105 + 573 WP_000779831.1 YbaY family lipoprotein -
H0E72_RS05190 1120136..1120447 - 312 WP_000409911.1 MGMT family protein -
H0E72_RS05200 1120826..1121179 + 354 WP_000878140.1 DUF1428 domain-containing protein -
H0E72_RS05205 1121220..1122770 - 1551 WP_012311818.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H0E72_RS05210 1122934..1123404 - 471 WP_000136192.1 YlaC family protein -
H0E72_RS05215 1123520..1124071 - 552 WP_000102561.1 maltose O-acetyltransferase -
H0E72_RS05220 1124243..1124461 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H0E72_RS05225 1124487..1124861 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H0E72_RS05230 1125407..1128556 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
H0E72_RS05235 1128579..1129772 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T168077 WP_001291435.1 NZ_CP059003:c1124461-1124243 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T168077 NZ_CP075627:3951678-3952055 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGTATCTCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAT
CCGACTGCTGGACCAGCATTATGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AATGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT168077 WP_000344800.1 NZ_CP059003:c1124861-1124487 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT168077 NZ_CP075627:3951257-3951631 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGAAATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGGCACAGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References