Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 1089935..1090772 Replicon chromosome
Accession NZ_CP059003
Organism Escherichia coli strain 2D

Toxin (Protein)


Gene name itaT Uniprot ID V0SED1
Locus tag H0E72_RS05050 Protein ID WP_000227787.1
Coordinates 1089935..1090477 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID B1LJI1
Locus tag H0E72_RS05055 Protein ID WP_001353405.1
Coordinates 1090461..1090772 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0E72_RS05030 1085475..1086386 - 912 WP_000705871.1 2-dehydropantoate 2-reductase -
H0E72_RS05035 1086554..1087045 + 492 WP_001138904.1 nucleotide binding protein YajQ -
H0E72_RS05040 1087173..1088537 - 1365 WP_001000978.1 MFS transporter -
H0E72_RS05045 1088944..1089879 + 936 WP_012311701.1 sel1 repeat family protein -
H0E72_RS05050 1089935..1090477 - 543 WP_000227787.1 GNAT family N-acetyltransferase Toxin
H0E72_RS05055 1090461..1090772 - 312 WP_001353405.1 DUF1778 domain-containing protein Antitoxin
H0E72_RS05060 1090957..1091847 - 891 WP_000971336.1 heme o synthase -
H0E72_RS05065 1091859..1092188 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
H0E72_RS05070 1092188..1092802 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
H0E72_RS05075 1092792..1094783 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
H0E72_RS05080 1094805..1095752 - 948 WP_001239436.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(19-96)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19946.24 Da        Isoelectric Point: 8.8951

>T168076 WP_000227787.1 NZ_CP059003:c1090477-1089935 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHRALPIKGVYLDADPAAINFYTRLGFVQLSA
RPNVFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T168076 NZ_CP075627:c3783045-3782638 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11370.09 Da        Isoelectric Point: 10.4825

>AT168076 WP_001353405.1 NZ_CP059003:c1090772-1090461 [Escherichia coli]
MPGKTVALPDIDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT168076 NZ_CP075627:c3783292-3783026 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GE43

Antitoxin

Source ID Structure
AlphaFold DB A0A829G7G3

References