Detailed information of TA system TA168075

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 933705..934411
• Replicon: chromosome
• Accession: NZ_CP059003
• Organism: Escherichia coli strain 2D

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: V0SXW1
• Locus tag: H0E72_RS04310
• Protein ID: WP_000854677.1
• Coordinates: 934043..934411 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: V0SU84
• Locus tag: H0E72_RS04305
• Protein ID: WP_000065824.1
• Coordinates: 933705..934022 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0E72_RS04280	930760..931973	+	1214	WP_085949836.1	IS3 family transposase	-
H0E72_RS04285	932184..932420	+	237	Protein_828	DUF905 domain-containing protein	-
H0E72_RS04290	932506..932964	+	459	WP_000211834.1	antirestriction protein	-
H0E72_RS04295	932976..933455	+	480	WP_000438147.1	RadC family protein	-
H0E72_RS04300	933464..933685	+	222	WP_001220307.1	DUF987 domain-containing protein	-
H0E72_RS04305	933705..934022	+	318	WP_000065824.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H0E72_RS04310	934043..934411	+	369	WP_000854677.1	TA system toxin CbtA family protein	Toxin
H0E72_RS04315	935204..935818	+	615	WP_001019920.1	YagU family protein	-
H0E72_RS04320	936067..936396	-	330	WP_001303809.1	YkgJ family cysteine cluster protein	-
H0E72_RS04325	936709..937419	-	711	WP_001361803.1	fimbrial chaperone EcpE	-
H0E72_RS04330	937388..939031	-	1644	WP_001265639.1	fimbrial adhesin EcpD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	yagV/ecpE / yagW/ecpD / yagX/ecpC / yagY/ecpB / yagZ/ecpA / ykgK/ecpR / fdeC	924005..962742	38737

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (5-104)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13687.89 Da
• Isoelectric Point: 8.2671

>T168075 WP_000854677.1 NZ_CP059003:934043-934411 [Escherichia coli]
MKTLPATTPQAAKPCLSPVSVWQMLLTRLLEQHYGLMLSDTPFSDETVIKEHIDAGITLANAVNFLVEKYELVRIDRNGF
TSQVQAPYLTTTDILQARKACGLMSRCSYREVSNIVLSRSRL

Nucleotide

• Length: 369 bp

>T168075 NZ_CP075627:c3646761-3646426 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11964.46 Da
• Isoelectric Point: 6.3163

>AT168075 WP_000065824.1 NZ_CP059003:933705-934022 [Escherichia coli]
MSNITWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAEYRKLDETFPHFISQMESMLTTGEMNPRHAHCVTLYH
DGFTCEADTLGSCGYVYIVVYSTQR

Nucleotide

• Length: 318 bp

>AT168075 NZ_CP075627:c3647009-3646761 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: V0SXW1

Antitoxin

• AlphaFold DB: V0SU84