Detailed information of TA system TA168073

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 390706..391301
• Replicon: chromosome
• Accession: NZ_CP059003
• Organism: Escherichia coli strain 2D

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: H0E72_RS01860
• Protein ID: WP_000239581.1
• Coordinates: 390951..391301 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: H0E72_RS01855
• Protein ID: WP_001223213.1
• Coordinates: 390706..390957 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0E72_RS01845	386371..390150	+	3780	WP_000060872.1	autotransporter assembly complex protein TamB	-
H0E72_RS01850	390153..390494	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
H0E72_RS01855	390706..390957	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
H0E72_RS01860	390951..391301	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
H0E72_RS01865	391380..391910	-	531	WP_000055075.1	inorganic diphosphatase	-
H0E72_RS01870	392220..393176	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
H0E72_RS01875	393309..394811	+	1503	WP_001095664.1	sugar ABC transporter ATP-binding protein	-
H0E72_RS01880	394825..395847	+	1023	WP_012311695.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T168073 WP_000239581.1 NZ_CP059003:390951-391301 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T168073 NZ_CP075627:c2658034-2657931 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT168073 WP_001223213.1 NZ_CP059003:390706-390957 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT168073 NZ_CP075627:2657893-2658164 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7