Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 29752..30277 Replicon plasmid pZZW20-88K
Accession NZ_CP058962
Organism Klebsiella pneumoniae strain ZZW20

Toxin (Protein)


Gene name ccdB Uniprot ID D4HQE7
Locus tag H0E90_RS28305 Protein ID WP_013023785.1
Coordinates 29972..30277 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag H0E90_RS28300 Protein ID WP_001568025.1
Coordinates 29752..29970 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0E90_RS28280 25017..25997 + 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
H0E90_RS28285 26439..27119 - 681 WP_032445676.1 hypothetical protein -
H0E90_RS28290 27413..28441 - 1029 WP_032445668.1 Abi family protein -
H0E90_RS28295 28602..29183 - 582 WP_072310991.1 hypothetical protein -
H0E90_RS28300 29752..29970 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H0E90_RS28305 29972..30277 + 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB Toxin
H0E90_RS28310 30446..30841 + 396 WP_164530166.1 hypothetical protein -
H0E90_RS28315 30868..31191 + 324 WP_004197641.1 hypothetical protein -
H0E90_RS28320 31188..32204 + 1017 WP_164530167.1 hypothetical protein -
H0E90_RS28325 32402..33187 + 786 WP_046664219.1 site-specific integrase -
H0E90_RS28330 33636..34391 + 756 WP_001568031.1 replication initiation protein RepE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-3 / blaTEM-1B / mcr-8 - 1..88061 88061


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11615.30 Da        Isoelectric Point: 6.4672

>T168060 WP_013023785.1 NZ_CP058962:29972-30277 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T168060 NZ_CP075605:27882-28157 [Enterococcus faecalis]
ATGCTTGAAATATTTTACACGAACCAATTCAAAAAAGACTTTAAGAAAGCAAAGAAACAAGGAAAGAATTTAGAGAAATT
AAAAGAAGTATTAGTACTTTTACAAGAACAGCAAACATTGCCGCCAAAGTATAAAGACCATGCACTAACAGGAAATTATA
TAGGTACACGAGAGTGCCATATTGAACCAGATTGGTTACTGATTTATAAAATTGACGGTGATAAATTGATCCTAACTTTA
GCCCGAATAGGCTCACATAGTGAATTATTTCGATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT168060 WP_001568025.1 NZ_CP058962:29752-29970 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT168060 NZ_CP075605:27623-27889 [Enterococcus faecalis]
GTGGCTACTACAAAAAAGAAACCTATACATGTAAATGTTGATGAAAATTTAAAAGAAGAAGCAGAACAATTATTTGATGA
TTTAGGGTTAAATATGACAAGTGCAATTACGATTTTCTTAAAACAGTCTATTAATGAGCAAGCAATTCCTTTTATGATTA
ATAAGGGAAACAAAGAGACTCTACAAGCATTAAAAGACATTAAAGAAGGAAATGTTCATGGTGGATTTTCTTCCGTGGAG
GATTTAATGGAGGATTTAAATGCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4ZZW6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References