Detailed information of TA system TA168058

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 24132..24775
• Replicon: plasmid pZZW20-108K
• Accession: NZ_CP058961
• Organism: Klebsiella pneumoniae strain ZZW20

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: H0E90_RS27610
• Protein ID: WP_001044770.1
• Coordinates: 24132..24548 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: H0E90_RS27615
• Protein ID: WP_001261282.1
• Coordinates: 24545..24775 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0E90_RS27585	19737..20621	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
H0E90_RS27590	20980..21522	-	543	WP_004896925.1	plasmid replication DNA-binding protein	-
H0E90_RS27595	21589..22341	-	753	Protein_25	replication initiation protein RepM	-
H0E90_RS27600	22407..23111	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H0E90_RS27605	23162..24058	-	897	Protein_27	AAA family ATPase	-
H0E90_RS27610	24132..24548	-	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
H0E90_RS27615	24545..24775	-	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
H0E90_RS27620	24732..25192	+	461	Protein_30	hypothetical protein	-
H0E90_RS27625	25362..25580	+	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	-
H0E90_RS27630	25582..25887	+	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	-
H0E90_RS27635	26074..27078	+	1005	WP_001568027.1	hypothetical protein	-
H0E90_RS27640	27262..28041	+	780	WP_001568028.1	site-specific integrase	-
H0E90_RS27645	28099..28356	-	258	WP_000764642.1	hypothetical protein	-
H0E90_RS27650	28514..28918	+	405	WP_003031976.1	IS66 family insertion sequence hypothetical protein	-
H0E90_RS27655	28915..29262	+	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	qnrB4 / blaDHA-1 / sul1 / armA / msr(E) / mph(E) / blaTEM-1C / tet(A) / aac(6')-Ib-cr / ARR-3 / dfrA27 / aadA16 / qacE / mph(A) / floR / aac(3)-IId / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2	-	1..108036	108036

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T168058 WP_001044770.1 NZ_CP058961:c24548-24132 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T168058 NZ_CP075604:3148436-3148579 [Enterococcus faecalis]
ATGAATGTAAGTACTAAAATCTATGAAAGGAGAGGCCTTTTGTCTATCGCAGAAGCTTTAGCTCTGATGATTAGTTTCGG
TTCTTTTATCGCAACGTTAATCTTCGGAATCCTAGAGGCTGTTAAAGAAGACAATAAAAAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT168058 WP_001261282.1 NZ_CP058961:c24775-24545 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT168058 NZ_CP075604:c3148339-3148138 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTGTAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGCGCAATCAAAGCAATGGTAAACATACCAA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3