Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5434603..5435228 Replicon chromosome
Accession NZ_CP058960
Organism Klebsiella pneumoniae strain ZZW20

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag H0E90_RS27100 Protein ID WP_002882817.1
Coordinates 5434603..5434986 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag H0E90_RS27105 Protein ID WP_004150355.1
Coordinates 5434986..5435228 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0E90_RS27085 5431969..5432871 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
H0E90_RS27090 5432868..5433503 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
H0E90_RS27095 5433500..5434429 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
H0E90_RS27100 5434603..5434986 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
H0E90_RS27105 5434986..5435228 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
H0E90_RS27110 5435433..5436350 + 918 WP_002882812.1 alpha/beta hydrolase -
H0E90_RS27115 5436364..5437305 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
H0E90_RS27120 5437350..5437787 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
H0E90_RS27125 5437784..5438644 - 861 WP_002882807.1 virulence factor BrkB family protein -
H0E90_RS27130 5438638..5439237 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T168057 WP_002882817.1 NZ_CP058960:c5434986-5434603 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T168057 NZ_CP075604:3148065-3148205 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAGCATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCGCTGATTGTGAAATTGCTTAAAAATGACAAGAAAAAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT168057 WP_004150355.1 NZ_CP058960:c5435228-5434986 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT168057 NZ_CP075604:c3148339-3148138 [Enterococcus faecalis]
GTTTTTTTAGGTAATTGTGCTGTAATAGCAATGAAAAGAGAGATATGCGCCAACATACCTCTCTGATGTAGAGCCGTTTA
AGACGGTGACCTTTTTGATTATTTAAAAATAACCGTACTGGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCA
ATTTCACAATCAGCGCAATCAAAGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References