Detailed information of TA system TA168052

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2718930..2720518
• Replicon: chromosome
• Accession: NZ_CP058960
• Organism: Klebsiella pneumoniae strain ZZW20

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A0H3GN97
• Locus tag: H0E90_RS13550
• Protein ID: WP_004199851.1
• Coordinates: 2718930..2720252 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: H0E90_RS13555
• Protein ID: WP_002904790.1
• Coordinates: 2720252..2720518 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H0E90_RS13535	2713995..2716496	+	2502	WP_004199853.1	fimbrial biogenesis outer membrane usher protein	-
H0E90_RS13540	2716484..2717488	+	1005	WP_004199852.1	type 1 fimbrial protein	-
H0E90_RS13545	2717621..2718913	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
H0E90_RS13550	2718930..2720252	-	1323	WP_004199851.1	type II toxin-antitoxin system HipA family toxin	Toxin
H0E90_RS13555	2720252..2720518	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
H0E90_RS13560	2720784..2721110	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
H0E90_RS13565	2721107..2721607	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
H0E90_RS13570	2721585..2722964	-	1380	WP_004199850.1	purine permease	-
H0E90_RS13575	2723310..2724464	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
H0E90_RS13580	2724445..2725368	-	924	WP_004199849.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49128.44 Da
• Isoelectric Point: 7.2487

>T168052 WP_004199851.1 NZ_CP058960:c2720252-2718930 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPLEQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T168052 NZ_CP075604:c3141022-3140681 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCAAGGACAAGTAGTGATCTCACAA
CTGAAGTCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT168052 WP_002904790.1 NZ_CP058960:c2720518-2720252 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT168052 NZ_CP075604:c3141252-3141022 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A0H3GN97

Antitoxin

• AlphaFold DB: A0A0H3GXD8