Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 736488..737263 Replicon chromosome
Accession NZ_CP058960
Organism Klebsiella pneumoniae strain ZZW20

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A1Q4Q548
Locus tag H0E90_RS03675 Protein ID WP_004150910.1
Coordinates 736778..737263 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID W8UEW1
Locus tag H0E90_RS03670 Protein ID WP_004150912.1
Coordinates 736488..736781 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H0E90_RS03650 731696..732298 - 603 WP_002916735.1 short chain dehydrogenase -
H0E90_RS03655 732396..733307 + 912 WP_004188550.1 LysR family transcriptional regulator -
H0E90_RS03660 733308..734456 - 1149 WP_004150915.1 PLP-dependent aspartate aminotransferase family protein -
H0E90_RS03665 734467..735843 - 1377 WP_004217775.1 cystathionine beta-synthase -
H0E90_RS03670 736488..736781 + 294 WP_004150912.1 DUF1778 domain-containing protein Antitoxin
H0E90_RS03675 736778..737263 + 486 WP_004150910.1 GNAT family N-acetyltransferase Toxin
H0E90_RS03680 737861..738841 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
H0E90_RS03685 739167..739760 + 594 WP_004188553.1 hypothetical protein -
H0E90_RS03690 739857..740073 + 217 Protein_725 transposase -
H0E90_RS03695 740679..741551 + 873 WP_004188557.1 ParA family protein -
H0E90_RS03700 741551..741934 + 384 WP_004150906.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(41-143)

Antitoxin

Vibrio phage ICP1, Orf50

(3-95)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17551.59 Da        Isoelectric Point: 8.8818

>T168042 WP_004150910.1 NZ_CP058960:736778-737263 [Klebsiella pneumoniae]
MISAPEPLHAGHILTPFCCGIDSMDHWLKQRAMKNQVTGASRTFVCCDDAKVMAYYSLASSAVTTNTAPGRFRRNMPAPI
PVVVLGRLAVDKSLHGKGVGRALVRDAGLRVIQVAETIGIRGMLVHALSDEARDFYLRVGFEPSPMDPMMLMVTLRDLVN
A

Download         Length: 486 bp

>T168042 NZ_CP075595:c677998-677693 [Pseudomonas sp. RC3H12]
ATGACTGAAACGTTCCATGAGTTCGACGCCGCCGCCTATCTGACTTCCCCGGAAACCATCGCGCAGTTCATGACCGATGC
ATTGGAGACAGGGGATGCAGCCTACGTAGCAAAAGCCGTGGGCGTGGTGGCTCGCGCCAGGGGCATGAGTGAACTGGCAA
AGGAAACAGGCCTGTCTCGCGAGCAGCTTTACCGCTCGTTCAGTGAACACGGCAACCCAACGCTCAAATCGTTCCTGACT
GTAATGAAAGCGTTGGGAATAGACATGGCTGCCAGACCCCACTTATCCCAGCAAGGTGTAGGCTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10901.46 Da        Isoelectric Point: 6.3783

>AT168042 WP_004150912.1 NZ_CP058960:736488-736781 [Klebsiella pneumoniae]
MPAANSMSIKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPQAYQEFLARLDQAPAPN
AALRKTMQTPAPWEQEE

Download         Length: 294 bp

>AT168042 NZ_CP075595:c678293-677991 [Pseudomonas sp. RC3H12]
ATGAAAACCATCGAATCCAGCAGTTTCAGGCATTGGCTGATCGGGCTTCGGGATGCCACGGCAAGGGCCAGAATCGTCGC
TCGTGTCAACCGACTCAAGGAAGGGCTAGCGGGTGACGTTTCACCGGTCGGCCATGGCATGAGTGAGCTGAGAGTCCATT
ACGGCCCAGGCTATCGGGTGTATTTTCACCAATACGGCGATACCTTGATCATCCTGCTTTGCGGCGGCGACAAAAGCAGC
CAGCAACGGGACATCAAGGCAGCCCGGCAGATCCTGCAGTCGTGGAGGATGCAAAATGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1Q4Q548

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GVL4

References