Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3236492..3237128 Replicon chromosome
Accession NZ_CP058951
Organism Bacillus pumilus strain UAMX

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag HZ310_RS16805 Protein ID WP_003214169.1
Coordinates 3236492..3236842 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A267X601
Locus tag HZ310_RS16810 Protein ID WP_012008995.1
Coordinates 3236847..3237128 (-) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZ310_RS16765 3231525..3232124 - 600 WP_003214034.1 SpoIIE family protein phosphatase -
HZ310_RS16770 3232124..3232912 - 789 WP_058015397.1 RNA polymerase sigma factor SigB -
HZ310_RS16775 3232878..3233366 - 489 WP_119124437.1 anti-sigma B factor RsbW -
HZ310_RS16780 3233363..3233692 - 330 WP_061406832.1 anti-sigma factor antagonist -
HZ310_RS16785 3233752..3234759 - 1008 WP_180309523.1 PP2C family protein-serine/threonine phosphatase -
HZ310_RS16790 3234770..3235171 - 402 WP_003214085.1 anti-sigma regulatory factor -
HZ310_RS16795 3235174..3235542 - 369 WP_003214235.1 RsbT antagonist protein RsbS -
HZ310_RS16800 3235547..3236377 - 831 WP_180309524.1 RsbT co-antagonist protein RsbRA -
HZ310_RS16805 3236492..3236842 - 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
HZ310_RS16810 3236847..3237128 - 282 WP_012008995.1 hypothetical protein Antitoxin
HZ310_RS16815 3237421..3238602 - 1182 WP_180309525.1 alanine racemase -
HZ310_RS16820 3238738..3239754 - 1017 WP_144463289.1 outer membrane lipoprotein carrier protein LolA -
HZ310_RS16825 3239915..3240280 - 366 WP_144463287.1 holo-ACP synthase -
HZ310_RS16830 3240375..3240980 + 606 WP_180309526.1 rhomboid family intramembrane serine protease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T168032 WP_003214169.1 NZ_CP058951:c3236842-3236492 [Bacillus pumilus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T168032 NZ_CP075591:355743-356090 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATACCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCGATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10894.27 Da        Isoelectric Point: 5.2726

>AT168032 WP_012008995.1 NZ_CP058951:c3237128-3236847 [Bacillus pumilus]
MSESSATNEMKIRLPETLMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT168032 NZ_CP075591:356090-356389 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCAGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A267X601

References