Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3476568..3477222 Replicon chromosome
Accession NZ_CP058948
Organism Escherichia coli strain SY3626C5

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag HZ988_RS16835 Protein ID WP_000244777.1
Coordinates 3476568..3476975 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HZ988_RS16840 Protein ID WP_000354046.1
Coordinates 3476956..3477222 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HZ988_RS16815 3472525..3474258 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
HZ988_RS16820 3474264..3474974 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HZ988_RS16825 3474999..3475895 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
HZ988_RS16830 3476007..3476528 + 522 WP_001055874.1 flavodoxin FldB -
HZ988_RS16835 3476568..3476975 - 408 WP_000244777.1 toxin CptA Toxin
HZ988_RS16840 3476956..3477222 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HZ988_RS16845 3477465..3478445 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
HZ988_RS16850 3478641..3479300 - 660 WP_000250274.1 hemolysin III family protein -
HZ988_RS16855 3479464..3479775 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
HZ988_RS16860 3479820..3481253 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
HZ988_RS16865 3481310..3482053 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T168017 WP_000244777.1 NZ_CP058948:c3476975-3476568 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T168017 NZ_CP075566:c773536-773252 [Pseudomonas fluorescens]
ATGCGCGTTGAGTGGTTGCGTACTGCATCAAAGAACCTCGATGACGAAGCTGCATACATAGCGATGGAAAATCCAAAAGC
TGCAGCAGATTTCGTTCAAGCCATTCTCGCCAGCGTCGATCACCTTGCTCACTTTCCAGCAACAGGTCGTGAGGGTCGTT
TGCCTGGCACTCGTGAATGGGTACTGCCCGACCGACCGTATCTGATTCCCTATCGAGTGCGCCAAGGTCGCCTACAAGTG
CTTCGGGTCTTTCATACACGACGCCTTCCGCCCACTAATTGGTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT168017 WP_000354046.1 NZ_CP058948:c3477222-3476956 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT168017 NZ_CP075566:c773765-773526 [Pseudomonas fluorescens]
ATGTCCGTCATGTCCTTGCGCCTGCCAGACGAAATCGCCGATACCCTCGCCACTCTTTCCAAAGCCACAGGCCGCAGCAA
GTCGTTTCTTGCCGTGGATGCACTGCGCGAATACCTGGCCCGGGAAGCCTGGCAAATCGAAGAAATTCAAAAAGCACTGA
TAGAAGCCGACGCAGGGGATTTCGTAACGCCTGAAGAAGTGAATGCAATCGCCAACAAATGGATAGCCAATGCGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References