Detailed information of TA system TA167977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 53667..54403
• Replicon: plasmid pIncFIBK_IncFIIK
• Accession: NZ_CP058941
• Organism: Klebsiella pneumoniae strain BA39649

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: HBJ39_RS26890
• Protein ID: WP_003026803.1
• Coordinates: 53921..54403 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: HBJ39_RS26885
• Protein ID: WP_003026799.1
• Coordinates: 53667..53933 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ39_RS26840 (HBJ39_026830)	49729..50091	-	363	WP_004152100.1	arsenite efflux transporter metallochaperone ArsD	-
HBJ39_RS26845 (HBJ39_026835)	50141..50491	-	351	WP_004152101.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
HBJ39_RS26850 (HBJ39_026840)	50849..51118	+	270	WP_004152102.1	hypothetical protein	-
HBJ39_RS26855 (HBJ39_026845)	51106..51681	+	576	WP_160333958.1	hypothetical protein	-
HBJ39_RS26860 (HBJ39_026850)	51712..52206	+	495	WP_004152104.1	DNA-binding protein	-
HBJ39_RS26865 (HBJ39_026855)	52250..52618	+	369	WP_004152105.1	hypothetical protein	-
HBJ39_RS26870 (HBJ39_026860)	52652..52855	+	204	WP_004152106.1	HHA domain-containing protein	-
HBJ39_RS26875 (HBJ39_026865)	52904..53161	+	258	WP_004152107.1	hypothetical protein	-
HBJ39_RS26880 (HBJ39_026870)	53237..53491	+	255	WP_004152108.1	hypothetical protein	-
HBJ39_RS26885 (HBJ39_026875)	53667..53933	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
HBJ39_RS26890 (HBJ39_026880)	53921..54403	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
HBJ39_RS26895 (HBJ39_026885)	54611..55956	+	1346	Protein_57	ISNCY family transposase	-
HBJ39_RS26900 (HBJ39_026890)	56005..56403	+	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
HBJ39_RS26905 (HBJ39_026895)	56599..57945	+	1347	WP_020314316.1	ISNCY family transposase	-
HBJ39_RS29720	58106..58237	+	132	WP_004218042.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	catA1	-	1..208077	208077

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T167977 WP_003026803.1 NZ_CP058941:53921-54403 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T167977 NZ_CP075549:65749-66081 [Klebsiella pneumoniae]
ATGGTATCTCGTTTTGTGCCGGATGCTGGCGACCTTATCTGGATAAATTTTGATCCCGTGGAAGGACATGAGCAAGGAGG
GCATCGCCCCGCAGTCGTGCTGAGTCCGTTTGCATACAACAACAAAACAGGTTTGTTGCTGTGCGTACCTTGCACAACAA
AAGTAAAAGGCTATCCCTTTGAAGTTGAATTGCCAGGAGAACGCGATGGTGTAGCTCTTGCAGATCAAATAACGTGTGTA
GACTGGCGAGCCAGAAAAGTAGAAAAGAAGGGAAAGGTTGCCACCGGAGAATTGTCAGAAATCAGAGCTAAAGCAAAAGC
CTTAATCGGTTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT167977 WP_003026799.1 NZ_CP058941:53667-53933 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT167977 NZ_CP075549:65504-65749 [Klebsiella pneumoniae]
ATGTCACAGGCAACGTTCAAGAAATGGGGTAACCAGGCAGGAATTCGTTTTCCGGCAGCGGTGATGAAAGCAGCAGCGCT
TAACGTAGATGATACGGTTGATATTGAGGTACAGGAAGGGCGGATTGTACTCATACCAACGAAAGAAAAGACCTATTCAC
TCGATGCCTTATTATCCGGCATCACCGAAGAAAACATGCATAACAAAGCTGACTTCGGCAAACCGACAGGGAAGGAAATG
CTCTGA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66