Detailed information of TA system TA167964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1804951..1805576
• Replicon: chromosome
• Accession: NZ_CP058940
• Organism: Klebsiella pneumoniae strain BA39649

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HBJ39_RS08805
• Protein ID: WP_040182550.1
• Coordinates: 1804951..1805334 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: J2DFR0
• Locus tag: HBJ39_RS08810
• Protein ID: WP_004150355.1
• Coordinates: 1805334..1805576 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ39_RS08790 (1802317)	1802317..1803219	+	903	WP_002882822.1	formate dehydrogenase subunit beta	-
HBJ39_RS08795 (1803216)	1803216..1803851	+	636	WP_002882818.1	formate dehydrogenase cytochrome b556 subunit	-
HBJ39_RS08800 (1803848)	1803848..1804777	+	930	WP_004150358.1	formate dehydrogenase accessory protein FdhE	-
HBJ39_RS08805 (1804951)	1804951..1805334	-	384	WP_040182550.1	type II toxin-antitoxin system VapC family toxin	Toxin
HBJ39_RS08810 (1805334)	1805334..1805576	-	243	WP_004150355.1	CopG family transcriptional regulator	Antitoxin
HBJ39_RS08815 (1805781)	1805781..1806698	+	918	WP_023302328.1	alpha/beta hydrolase	-
HBJ39_RS08820 (1806712)	1806712..1807653	-	942	WP_004178031.1	fatty acid biosynthesis protein FabY	-
HBJ39_RS08825 (1807698)	1807698..1808135	-	438	WP_002882809.1	D-aminoacyl-tRNA deacylase	-
HBJ39_RS08830 (1808132)	1808132..1808992	-	861	WP_004146232.1	virulence factor BrkB family protein	-
HBJ39_RS08835 (1808986)	1808986..1809585	-	600	WP_004151865.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (16-47)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14376.67 Da
• Isoelectric Point: 9.5352

>T167964 WP_040182550.1 NZ_CP058940:c1805334-1804951 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLKLITRNTKDFAGIPGVVTPYEIHPE

Nucleotide

• Length: 384 bp

>T167964 NZ_CP075548:353358-353705 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGACGATTACTATGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9567.83 Da
• Isoelectric Point: 4.3509

>AT167964 WP_004150355.1 NZ_CP058940:c1805576-1805334 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Nucleotide

• Length: 243 bp

>AT167964 NZ_CP075548:353705-354004 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3GGU9